| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 531 | Amedibacillus | AGGGTGTGAAGCGTTGGT | TGCATACGCCTGTCCATCG | 59.08 | 60.23 | 175 | 74 |
100.00%
|
50.00%
|
| Primer 532 | Amedibacillus | AGCTACGGGAAATCGCAATGA | CCAAGACGCTCAAAGGTCCA | 60.13 | 60.25 | 205 | 74 |
100.00%
|
50.00%
|
| Primer 533 | Amedibacillus | AGCTACGGGAAATCGCAATGA | ACCAAGACGCTCAAAGGTCC | 60.13 | 60.25 | 206 | 74 |
100.00%
|
50.00%
|
| Primer 534 | Amedibacillus | TGCAAACACCGAAGCGTG | AAACACCCGAGCAGGCAT | 59.29 | 59.56 | 206 | 74 |
100.00%
|
50.00%
|
| Primer 535 | Amedibacillus | AGCGGTGGAGAAATGCGAT | TTGCCCTTGGGTGTGCAT | 59.78 | 59.80 | 206 | 74 |
100.00%
|
50.00%
|
| Primer 536 | Amedibacillus | ACCAAAGCACAGGAGGAGG | CGAAGTCATCGCCTTCATCA | 59.24 | 58.07 | 181 | 74 |
100.00%
|
50.00%
|
| Primer 537 | Amedibacillus | CCGCAGGCACAGAAACAA | TGCTAATCCCCAATCCTCTCG | 58.57 | 59.31 | 200 | 74 |
100.00%
|
50.00%
|
| Primer 538 | Amedibacillus | TCGTACGGCAATGACGATGA | ACCGACGAGTGGGATAAGC | 59.55 | 59.19 | 199 | 74 |
100.00%
|
50.00%
|
| Primer 539 | Amedibacillus | TGCGGTTGCCTGTGATCA | TGCTTGCTACCGCTTTTGC | 59.57 | 59.71 | 158 | 74 |
100.00%
|
50.00%
|
| Primer 540 | Amedibacillus | TGCGGTTGCCTGTGATCA | AGCTGTGCACTAACCGGA | 59.57 | 58.53 | 273 | 74 |
100.00%
|
50.00%
|