| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 541 | Amedibacillus | TGCGCATGCCCACTATCA | TGAACAACCTCGCCTGCA | 59.41 | 59.49 | 282 | 74 |
100.00%
|
50.00%
|
| Primer 542 | Amedibacillus | GGCTGCTAATGCTCACGGA | GGCAATTCCAAACGGCTCA | 60.15 | 59.04 | 201 | 74 |
100.00%
|
50.00%
|
| Primer 543 | Amedibacillus | TGCGATGCACAGGGTGAT | ACATGGCGATGCTGTTGC | 59.33 | 59.12 | 269 | 74 |
100.00%
|
50.00%
|
| Primer 544 | Amedibacillus | GGCGATGAAGGAAGGTTGC | CATGCCACATCAAGCGTTGA | 59.20 | 59.48 | 152 | 74 |
100.00%
|
50.00%
|
| Primer 545 | Amedibacillus | TTACGCCTAGTGCAGAGCG | CCGCAACAATCGCAGCAT | 59.86 | 59.51 | 240 | 74 |
100.00%
|
50.00%
|
| Primer 546 | Amedibacillus | CAAGGGCTTGCTGAGGTGA | GCAAGCCTGCGACAATCA | 59.93 | 58.73 | 190 | 74 |
100.00%
|
50.00%
|
| Primer 547 | Amedibacillus | CACCGGAACAACTGCAACG | TCACGACCGCTCACTGTTC | 60.01 | 60.01 | 268 | 74 |
100.00%
|
50.00%
|
| Primer 548 | Amedibacillus | AGCTTGCTCGGTGTTTGGA | AGCATTGCACCGCTGATG | 59.85 | 58.81 | 245 | 73 |
100.00%
|
100.00%
|
| Primer 549 | Amedibacillus | AAGTGAGCGTGCCAATGC | TTACCGGTTCCGATTGCCC | 59.04 | 60.08 | 298 | 73 |
100.00%
|
100.00%
|
| Primer 550 | Amedibacillus | AGTTTGCGTGCTACCGGA | CGGAACAAAGCTGCCAACC | 59.26 | 60.01 | 247 | 73 |
100.00%
|
100.00%
|