| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Azotobacter | ATTTCCGCTCTGACCCTGG | TTTTCCAGCATGCGCAGG | 59.40 | 59.04 | 218 | 142 |
100.00%
|
100.00%
|
| Primer 2 | Azotobacter | AAAACAGTGCGCTGCTGC | CAGATGGCGTTCGATGTGC | 59.97 | 59.65 | 251 | 121 |
100.00%
|
100.00%
|
| Primer 3 | Azotobacter | TGCTACGACAACGCCTGT | TGTATCCCTGCGTGCTGTG | 59.27 | 60.08 | 160 | 113 |
100.00%
|
100.00%
|
| Primer 4 | Azotobacter | GCGTGACTTTTGGCAGAGC | AGCGCATATTGCAGCAGC | 60.08 | 59.28 | 260 | 111 |
100.00%
|
100.00%
|
| Primer 5 | Azotobacter | ATGCTTGAGAGGCGTCTGG | TCCATGCACCAGTACGCTG | 59.78 | 60.08 | 225 | 109 |
100.00%
|
100.00%
|
| Primer 6 | Azotobacter | ACTACCTGGACAAGCGCAC | GGCATCGTTCAGGCAATGC | 60.00 | 60.23 | 191 | 109 |
100.00%
|
100.00%
|
| Primer 7 | Azotobacter | ACTACCTGGACAAGCGCAC | TCGGTGATGGCCTCTTTGC | 60.00 | 60.38 | 166 | 109 |
100.00%
|
100.00%
|
| Primer 8 | Azotobacter | TCGTAGATGGGCATCGTGC | ATCACCGTGTACCCCATGC | 59.93 | 59.78 | 163 | 103 |
100.00%
|
100.00%
|
| Primer 9 | Azotobacter | TCGTAGATGGGCATCGTGC | TGCTGACGCTCGATGTAGC | 59.93 | 60.23 | 205 | 103 |
100.00%
|
100.00%
|
| Primer 10 | Azotobacter | GCCGTTGCCCTTCGAATTC | ATTGTCTCCCACGGACAGC | 59.86 | 59.70 | 202 | 102 |
100.00%
|
100.00%
|