| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Azotobacter | AAGCTGTTCGAACGGGACC | AGCACCGAGGAAACGAAGG | 60.30 | 60.00 | 170 | 100 |
100.00%
|
100.00%
|
| Primer 22 | Azotobacter | AAAACAGTGCGCTGCTGC | ACTGCCGTCCGTTCAGATG | 59.97 | 60.08 | 264 | 98 |
100.00%
|
100.00%
|
| Primer 23 | Azotobacter | TGTGTTATCGGCAGGGCAC | TTCCGAGAATCCCTGCAGG | 60.38 | 59.09 | 209 | 98 |
100.00%
|
100.00%
|
| Primer 24 | Azotobacter | AAGGAGAATGGCGTCCGTG | ACGCAGAGATTGAGGGCTG | 60.08 | 59.78 | 188 | 97 |
100.00%
|
100.00%
|
| Primer 25 | Azotobacter | AAGGAGAATGGCGTCCGTG | AGCAGTTCGTCCTTGCTGG | 60.08 | 60.30 | 161 | 97 |
100.00%
|
100.00%
|
| Primer 26 | Azotobacter | TTCTGGATCGAAGCGCTGG | ATCGAGGCGATCACCATGC | 60.15 | 60.30 | 161 | 97 |
100.00%
|
100.00%
|
| Primer 27 | Azotobacter | TCTCGAGATGGTTCGCTGC | AGTTCGGCTCGGATCGTTC | 59.86 | 59.86 | 221 | 97 |
100.00%
|
100.00%
|
| Primer 28 | Azotobacter | TCAACGAGGTGCTGAGCAG | GCGCAACCGTTCGAGATTC | 60.01 | 59.94 | 158 | 97 |
100.00%
|
100.00%
|
| Primer 29 | Azotobacter | GTTTCGCCACCAGCAACTG | AGCGTGTCGAGGGAAAAGG | 60.01 | 60.00 | 211 | 97 |
100.00%
|
100.00%
|
| Primer 30 | Azotobacter | ATGGCCTGGTCGATCTTGC | AGGTGAGCTGGCCATTCAC | 60.15 | 60.00 | 282 | 97 |
100.00%
|
100.00%
|