| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 131 | Bacillus | CCGTGGACGCATTGAAGTG | TGAGCAGCTTGGCGAACA | 59.50 | 59.89 | 252 | 94 |
100.00%
|
13.10%
|
| Primer 132 | Bacillus | ACCAGACGCAATCCGCAA | TCAAGGACCGCACGAACA | 59.97 | 59.18 | 180 | 94 |
100.00%
|
15.48%
|
| Primer 133 | Bacillus | ACAGCGGTTCGATTGGCA | TGGAGAAATCGCACCACGT | 59.97 | 59.63 | 270 | 94 |
100.00%
|
15.48%
|
| Primer 134 | Bacillus | ACCAGACGCAATCCGCAA | CGAACAGGTCCACCAGCAA | 59.97 | 60.23 | 168 | 94 |
100.00%
|
15.48%
|
| Primer 135 | Bacillus | ACCAGACGCAATCCGCAA | AGTACCGCACGAACAGGTC | 59.97 | 59.71 | 177 | 94 |
100.00%
|
15.48%
|
| Primer 136 | Bacillus | GTTCTGAGCAAGGAGGCGT | TGTCCGTTAAGCGCTGCT | 60.00 | 59.66 | 219 | 94 |
100.00%
|
15.48%
|
| Primer 137 | Bacillus | TTCTGAGCAAGGAGGCGT | TGTCCGTTAAGCGCTGCT | 58.53 | 59.66 | 218 | 94 |
100.00%
|
15.48%
|
| Primer 138 | Bacillus | CCATGAAAGCGTACGTCCAC | GCAGCCCTTACGGTAGCAT | 59.28 | 59.85 | 168 | 94 |
100.00%
|
14.29%
|
| Primer 139 | Bacillus | TGTTGTTGGAAATCTCGTCGT | TGCGCTAACACCGTTAAACG | 58.17 | 59.49 | 154 | 94 |
100.00%
|
14.29%
|
| Primer 140 | Bacillus | CCCCACAGCAGATGGTTCA | TGCAACTTCAGCGATTCGT | 59.62 | 58.09 | 251 | 94 |
100.00%
|
14.29%
|