| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 121 | Bacillus | CCGTCCGAACAGCAATTGC | ACACCGGTGCTACCTTCTG | 60.15 | 59.33 | 268 | 95 |
100.00%
|
15.48%
|
| Primer 122 | Bacillus | AGGACGCGATATGACTGAACT | TCCCATGAGTCCAACTAGCTT | 58.97 | 58.45 | 197 | 94 |
100.00%
|
15.48%
|
| Primer 123 | Bacillus | GCGATTGTCCAACTTGCCC | CGCTCTCCTCCAAAGCCTT | 59.79 | 59.70 | 213 | 94 |
100.00%
|
14.29%
|
| Primer 124 | Bacillus | CGGGTGAAGGGCTTGTTGA | TGAGCAGCTTGGCGAACA | 60.23 | 59.89 | 220 | 94 |
100.00%
|
15.48%
|
| Primer 125 | Bacillus | CGGGAGGACAAAAGCAACG | ATTGTTGCGCACGCTCAC | 59.43 | 59.75 | 291 | 94 |
100.00%
|
14.29%
|
| Primer 126 | Bacillus | GCCCTGGTCCGAAAGATCA | ACCGTCGTTTGCATCCCA | 59.40 | 59.57 | 161 | 94 |
100.00%
|
14.29%
|
| Primer 127 | Bacillus | TGAAGCGAGACAAATTGCAGC | CGAGCATATCGCCAGCTTC | 60.07 | 58.85 | 260 | 94 |
100.00%
|
15.48%
|
| Primer 128 | Bacillus | ACTCCTCCTGCTGCTGTTC | ATGCCCATACTGCGTGCA | 59.32 | 59.73 | 197 | 94 |
100.00%
|
15.48%
|
| Primer 129 | Bacillus | CGTACTTGTGCAGCAACTGG | GCTGTATGAGCTTTCTCGCC | 59.76 | 59.07 | 206 | 94 |
100.00%
|
15.48%
|
| Primer 130 | Bacillus | ACTCCTCCTGCTGCTGTTC | ATGCCCATACTGCGTGCA | 59.32 | 59.73 | 197 | 94 |
100.00%
|
15.48%
|