| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 101 | Bacillus | ACTCCATCGCCAGTTATGCA | TGGGTGCACTTGGTCAAAGA | 59.46 | 59.74 | 152 | 98 |
100.00%
|
15.48%
|
| Primer 102 | Bacillus | TTCGGCAAAAGCACAGGC | TCAGCGCTTCAAAGGCTGT | 59.27 | 60.23 | 167 | 97 |
100.00%
|
15.48%
|
| Primer 103 | Bacillus | AGGACGCGATATGACTGAACT | GTCCCATGAGTCCAACTAGCT | 58.97 | 59.17 | 198 | 97 |
100.00%
|
15.48%
|
| Primer 104 | Bacillus | GCCGAAAGCACTAGGTGGT | TGTGAAACGCCAGCGACA | 60.00 | 60.20 | 225 | 97 |
100.00%
|
14.29%
|
| Primer 105 | Bacillus | ACGCTCAGCAGGAAATCCA | TGCGATGACGAAAGCGGA | 59.32 | 59.74 | 256 | 97 |
100.00%
|
13.10%
|
| Primer 106 | Bacillus | GCGGTTTAACGGAAGAAGGC | TAACCCTGCCACGCAATCC | 59.83 | 60.38 | 218 | 97 |
100.00%
|
13.10%
|
| Primer 107 | Bacillus | ACGGTCAGGCTGCTTTAGG | CACCTCCGCAGTTTCTTGC | 59.70 | 59.42 | 203 | 97 |
100.00%
|
16.67%
|
| Primer 108 | Bacillus | AGGACGCGATATGACTGAACT | ACCTTCTTGTAAGCCACCTCT | 58.97 | 58.66 | 175 | 96 |
100.00%
|
15.48%
|
| Primer 109 | Bacillus | TTTCGCGTTACAGGGGACG | ATTCTGCCTCTGGTGCTCT | 60.37 | 58.31 | 166 | 96 |
100.00%
|
15.48%
|
| Primer 110 | Bacillus | TGCGTGCGGTAAACTGGA | CATCCCTTCGCCACCTTGT | 59.58 | 60.00 | 209 | 96 |
100.00%
|
13.10%
|