| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 81 | Bacillus | CCGTGGACGCATTGAAGTG | TGAGCAGCTTGGCGAACA | 59.50 | 59.89 | 252 | 99 |
100.00%
|
13.10%
|
| Primer 82 | Bacillus | TTTCGCGTTACAGGGGACG | CTGCCTCTGGTGCTCTAACAA | 60.37 | 60.00 | 163 | 99 |
100.00%
|
15.48%
|
| Primer 83 | Bacillus | TTTCGCGTTACAGGGGACG | TGCCTCTGGTGCTCTAACAA | 60.37 | 58.94 | 162 | 99 |
100.00%
|
15.48%
|
| Primer 84 | Bacillus | TCGAGCTCGATGTCCTTGC | GCGCTAAACGGCTTGAAGC | 59.86 | 60.51 | 273 | 99 |
100.00%
|
14.29%
|
| Primer 85 | Bacillus | TCAACTGGTGGAGCGACTG | TTGCAACACTAGCGCTTGC | 59.63 | 59.72 | 160 | 99 |
100.00%
|
13.10%
|
| Primer 86 | Bacillus | AATCGCTGCTGCCCTTGT | AAGCACCTGCAACCCCA | 59.97 | 58.98 | 206 | 99 |
100.00%
|
13.10%
|
| Primer 87 | Bacillus | AGGCGACGATGAGCAACT | ACAAGCGCACCTAAGCCA | 59.03 | 59.57 | 297 | 99 |
100.00%
|
14.29%
|
| Primer 88 | Bacillus | AAGGCGACGATGAGCAACT | ACAAGCGCACCTAAGCCA | 59.71 | 59.57 | 298 | 99 |
100.00%
|
15.48%
|
| Primer 89 | Bacillus | GGCGACGATGAGCAACTTG | ACAAGCGCACCTAAGCCA | 59.58 | 59.57 | 296 | 99 |
100.00%
|
15.48%
|
| Primer 90 | Bacillus | GAAGGCGACGATGAGCAAC | ACAAGCGCACCTAAGCCA | 59.29 | 59.57 | 299 | 99 |
100.00%
|
15.48%
|