| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1531 | Bacillus | CCAAGTGACTCGTGAGCCT | AGCCCAAACCCGATAGCAC | 59.33 | 60.08 | 225 | 58 |
100.00%
|
14.29%
|
| Primer 1532 | Bacillus | GCGGAGAGGCAACAAATCC | TGTGAAACGCCAGCGACA | 59.20 | 60.20 | 202 | 58 |
100.00%
|
14.29%
|
| Primer 1533 | Bacillus | TGCTCCAAATGCGAAACCG | ACGCTTTAACCTTGTCGCCT | 59.42 | 60.25 | 212 | 58 |
100.00%
|
15.48%
|
| Primer 1534 | Bacillus | TGGTGCTGAAGTGCGTGA | TCGATGCTCTCACGCGTT | 59.50 | 59.43 | 216 | 58 |
100.00%
|
15.48%
|
| Primer 1535 | Bacillus | AGCCGTGCAATTGCTGAAC | TGCTCCTCATCACCACGAAC | 59.71 | 60.04 | 287 | 58 |
100.00%
|
15.48%
|
| Primer 1536 | Bacillus | TGATGAAGTGGGGACTGCG | TCACCGTTTTCTGCACCCT | 59.70 | 59.47 | 210 | 58 |
100.00%
|
15.48%
|
| Primer 1537 | Bacillus | ATCCCAGCCGAAGAGCCTA | GCAAGTGCAACGTCGTGA | 60.08 | 58.99 | 170 | 58 |
100.00%
|
14.29%
|
| Primer 1538 | Bacillus | TGGCGATGGTTCAGAAGGAA | TGCCTTCACCCAATTCCCT | 59.31 | 58.83 | 215 | 58 |
100.00%
|
16.67%
|
| Primer 1539 | Bacillus | GCAGAACAAATTGTTGCCGTT | TTCCATCCGGTTCGCTACG | 58.80 | 59.86 | 181 | 58 |
100.00%
|
14.29%
|
| Primer 1540 | Bacillus | CGGTGGCATCGGTATTGGA | ACGCGTTGCCAATCTCCT | 59.85 | 59.65 | 180 | 58 |
100.00%
|
15.48%
|