| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1541 | Bacillus | CGAAAGTAGCGGTGGAAAACA | CGACGAGATTCCTCATCCCC | 59.13 | 59.68 | 277 | 58 |
100.00%
|
15.48%
|
| Primer 1542 | Bacillus | CGAAAGTAGCGGTGGAAAACA | ACGACGAGATTCCTCATCCC | 59.13 | 58.97 | 278 | 58 |
100.00%
|
15.48%
|
| Primer 1543 | Bacillus | AGCTGCTCGTCGTGCAAT | ATGCTTCGCGTGCTACCT | 60.05 | 59.42 | 209 | 58 |
100.00%
|
15.48%
|
| Primer 1544 | Bacillus | TCCTTGGACTGCAGATGCC | GCATCCCCTAGCTTTTGACCT | 59.70 | 60.06 | 180 | 58 |
100.00%
|
14.29%
|
| Primer 1545 | Bacillus | CCTTGGACTGCAGATGCCT | GCATCCCCTAGCTTTTGACCT | 59.70 | 60.06 | 179 | 58 |
100.00%
|
14.29%
|
| Primer 1546 | Bacillus | TGGCGATGGTTCAGAAGGA | ATGCCTTCACCCAATTCCCT | 58.63 | 59.29 | 216 | 58 |
100.00%
|
16.67%
|
| Primer 1547 | Bacillus | ATGGCGATGGTTCAGAAGGA | TGCCTTCACCCAATTCCCT | 59.09 | 58.83 | 216 | 58 |
100.00%
|
16.67%
|
| Primer 1548 | Bacillus | TGGCATGCATGGGTTGTTG | AGGCCACCTACCTCTTCCT | 59.33 | 59.21 | 218 | 58 |
100.00%
|
16.67%
|
| Primer 1549 | Bacillus | AAAGCAACGTCGGTCCCT | TGCTGTACGCTCTTCCCCA | 59.17 | 60.91 | 216 | 58 |
100.00%
|
15.48%
|
| Primer 1550 | Bacillus | TGCACAAGAAGCAGCGGA | GTGCGGAATACTTTCGCTTGT | 59.89 | 59.54 | 171 | 58 |
100.00%
|
15.48%
|