| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2401 | Bacillus | TGGTTCGAGCAACTATGGGG | TCTCCCACGAGACGTTTTCG | 59.75 | 60.04 | 255 | 44 |
100.00%
|
15.48%
|
| Primer 2402 | Bacillus | TGGTTCGAGCAACTATGGGG | AAGTGTCCTCTCCCACGAGA | 59.75 | 59.89 | 263 | 44 |
100.00%
|
15.48%
|
| Primer 2403 | Bacillus | TTGCACAGCAAGTTCGCC | ATGAGCAGCTTGGCGAACA | 59.28 | 60.30 | 269 | 44 |
100.00%
|
14.29%
|
| Primer 2404 | Bacillus | AGCCGTGCAATTGCTGAAC | GCTCCTCATCACCACGAACA | 59.71 | 60.04 | 286 | 44 |
100.00%
|
15.48%
|
| Primer 2405 | Bacillus | CCCGTATGATGACGAGCCAA | TGCATGGACCGCATCATGA | 59.90 | 59.78 | 202 | 44 |
100.00%
|
14.29%
|
| Primer 2406 | Bacillus | AGTGACAGGGTGGGGAGAT | TCGGTGTTTCCATTGCCCA | 59.52 | 59.85 | 164 | 44 |
100.00%
|
15.48%
|
| Primer 2407 | Bacillus | TAACGGCAGGGCTGGATT | TCGTCCACGGTGCATTGT | 58.59 | 59.58 | 236 | 43 |
100.00%
|
15.48%
|
| Primer 2408 | Bacillus | ACGACACCAATTGCAATGGC | ACCGGTTCCATTTGCGCT | 60.04 | 60.28 | 183 | 43 |
100.00%
|
15.48%
|
| Primer 2409 | Bacillus | CGACACCAATTGCAATGGCT | ACCGGTTCCATTTGCGCT | 59.76 | 60.28 | 182 | 43 |
100.00%
|
15.48%
|
| Primer 2410 | Bacillus | ACGTGAAAATGCACCGTGG | ATCGGCATCGTCCAACGT | 59.35 | 59.42 | 262 | 43 |
100.00%
|
14.29%
|