| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2421 | Bacillus | TGTGGGTGCACTGCAAATT | ACCACCCTAGACCGCTACT | 58.49 | 59.30 | 164 | 43 |
100.00%
|
14.29%
|
| Primer 2422 | Bacillus | CGACTGGTTTGTGGCTGGA | CCCGTGTCCACCACCAAAT | 60.23 | 60.23 | 236 | 43 |
100.00%
|
15.48%
|
| Primer 2423 | Bacillus | CTGTCGGCAGAACAAGAGTTT | CATCACTACCACCCTCCCCT | 58.79 | 60.33 | 229 | 43 |
100.00%
|
14.29%
|
| Primer 2424 | Bacillus | TGTCGGCAGAACAAGAGTTTT | ATCACTACCACCCTCCCCT | 58.36 | 58.88 | 227 | 43 |
100.00%
|
14.29%
|
| Primer 2425 | Bacillus | TGTCGGCAGAACAAGAGTTTT | TCATCACTACCACCCTCCCC | 58.36 | 60.33 | 229 | 43 |
100.00%
|
14.29%
|
| Primer 2426 | Bacillus | AAGCTGCTCGTCGTGCAA | ATGCTTCGCGTGCTACCT | 60.28 | 59.42 | 210 | 43 |
100.00%
|
15.48%
|
| Primer 2427 | Bacillus | AAGCTGCTCGTCGTGCAA | AATGCTTCGCGTGCTACCT | 60.28 | 60.08 | 211 | 43 |
100.00%
|
15.48%
|
| Primer 2428 | Bacillus | AACGAGCGCATCCAAACG | ACCATTGGTGCTGCGTCT | 59.14 | 59.57 | 266 | 43 |
100.00%
|
15.48%
|
| Primer 2429 | Bacillus | TGAAGGCGACGATGAGCA | AAGCGCACCTAAACCACCT | 59.03 | 59.55 | 298 | 43 |
100.00%
|
14.29%
|
| Primer 2430 | Bacillus | TGAAGGCGACGATGAGCAA | AGCGCACCTAAACCACCT | 59.71 | 58.84 | 297 | 43 |
100.00%
|
15.48%
|