| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 261 | Bacillus | TGCTGATCGTTGGGCTGA | ACCAATTACCCCAGCACCG | 58.93 | 60.00 | 280 | 86 |
100.00%
|
14.29%
|
| Primer 262 | Bacillus | TCCCAAAGCAAAACCGTGT | GGTTCGCCTGTTTTCGGATC | 58.11 | 59.55 | 154 | 86 |
100.00%
|
15.48%
|
| Primer 263 | Bacillus | TTCCCAAAGCAAAACCGTGT | GGTTCGCCTGTTTTCGGATC | 58.81 | 59.55 | 155 | 86 |
100.00%
|
15.48%
|
| Primer 264 | Bacillus | GCGGTTGTGTTATGAGCGG | AACTGGCTTGGGCTCTTCA | 59.58 | 59.15 | 190 | 86 |
100.00%
|
14.29%
|
| Primer 265 | Bacillus | GCGGTTGTGTTATGAGCGG | GGGCTCTTCAGCTGGAAAGT | 59.58 | 59.96 | 181 | 86 |
100.00%
|
13.10%
|
| Primer 266 | Bacillus | GCGGTTGTGTTATGAGCGG | GTCCCTCCAACTTCATGCCA | 59.58 | 59.96 | 163 | 86 |
100.00%
|
13.10%
|
| Primer 267 | Bacillus | TCTGAGCAAGGAGGCGTT | TGTCCGTTAAGCGCTGCT | 58.53 | 59.66 | 217 | 86 |
100.00%
|
15.48%
|
| Primer 268 | Bacillus | TTCTGAGCAAGGAGGCGTT | TGTCCGTTAAGCGCTGCT | 59.25 | 59.66 | 218 | 86 |
100.00%
|
15.48%
|
| Primer 269 | Bacillus | AAATGGCGTGGGCTCACT | CCACCGCTTATCGCAGCTA | 59.56 | 59.93 | 269 | 86 |
100.00%
|
13.10%
|
| Primer 270 | Bacillus | ACGGTCAGGCTGCTTTAGG | CACCTCCGCAGTTTCTTGC | 59.70 | 59.42 | 203 | 86 |
100.00%
|
16.67%
|