| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 281 | Bacillus | CGGATCGATTCGAGTGCGT | TCTGCATTCCCTTGCGCA | 60.30 | 59.97 | 202 | 85 |
100.00%
|
15.48%
|
| Primer 282 | Bacillus | TGCGGACATTCATCGTCGT | TCTGTTTCCGATGCAGCTGT | 59.79 | 59.96 | 225 | 85 |
100.00%
|
15.48%
|
| Primer 283 | Bacillus | GCAAACGGACATGCGCAT | AACGCTTGCGCAACAGC | 59.82 | 60.02 | 161 | 85 |
100.00%
|
13.10%
|
| Primer 284 | Bacillus | GTATTGCGCACGCTGCAT | CAGCTTCGCGAAGTGCTTC | 59.60 | 59.87 | 195 | 85 |
100.00%
|
15.48%
|
| Primer 285 | Bacillus | CCGAAAGTAGCGGTGGAAAAC | CGACGAGATTCCTCATCCCC | 59.80 | 59.68 | 278 | 85 |
100.00%
|
15.48%
|
| Primer 286 | Bacillus | CCGAAAGTAGCGGTGGAAAAC | ACGACGAGATTCCTCATCCC | 59.80 | 58.97 | 279 | 85 |
100.00%
|
15.48%
|
| Primer 287 | Bacillus | ATGCCTCAGTCGATGCTGC | TCATCTGCTTCGTCTGCCC | 60.52 | 59.78 | 289 | 85 |
100.00%
|
15.48%
|
| Primer 288 | Bacillus | TGCGGTTGCTGGTCTCAT | ACGTTTCCATCCGCAACC | 59.25 | 58.34 | 180 | 85 |
100.00%
|
15.48%
|
| Primer 289 | Bacillus | TTCGGCAAAAGCACAGGC | AGCGCTTCAAAGGCTGTACT | 59.27 | 59.96 | 165 | 84 |
100.00%
|
15.48%
|
| Primer 290 | Bacillus | ACCAGACGCAATCCGCAA | TTCAAGGACCGCACGAACA | 59.97 | 59.86 | 181 | 84 |
92.86%
|
15.48%
|