| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 301 | Bacillus | TGGTGTTGGTGATGCGGT | CTTTCGCACCGATTGGCA | 59.49 | 58.74 | 152 | 84 |
100.00%
|
15.48%
|
| Primer 302 | Bacillus | TGCGTGCGGTAAACTGGA | ATCCCTTCGCCACCTTGT | 59.58 | 58.50 | 208 | 84 |
100.00%
|
13.10%
|
| Primer 303 | Bacillus | TTGCAACGCCTGTAGGGT | TCGCGATGGGCAGGTAAA | 59.17 | 59.02 | 274 | 84 |
100.00%
|
14.29%
|
| Primer 304 | Bacillus | TTGCAACGCCTGTAGGGT | TCTTCTTCGCGATGGGCAG | 59.17 | 60.15 | 280 | 84 |
100.00%
|
14.29%
|
| Primer 305 | Bacillus | TGGCGATGGTTCAGAAGGA | TGCACTCACTACGACGTTTTG | 58.63 | 59.14 | 239 | 84 |
100.00%
|
16.67%
|
| Primer 306 | Bacillus | AGCGCATTTAGCACGTCTTG | ACTGCTGGAACACGGATTTG | 59.56 | 58.76 | 246 | 84 |
100.00%
|
15.48%
|
| Primer 307 | Bacillus | TGCAGAGCGTCCACGTTT | CGCCTTTCTCTACAGCACG | 59.89 | 58.64 | 194 | 84 |
100.00%
|
15.48%
|
| Primer 308 | Bacillus | AAGGTCTCGGCATCGCTTT | ACGGAATTGCTGAGCGGT | 59.70 | 59.65 | 158 | 84 |
100.00%
|
13.10%
|
| Primer 309 | Bacillus | AGAAGGAAATCGCAGGGGAAA | TCACGCACAACCTCAGCA | 59.65 | 59.50 | 259 | 83 |
100.00%
|
13.10%
|
| Primer 310 | Bacillus | GCTGGCGAGCAAGGATTTG | TGCTCCTGCACGATCCATG | 59.86 | 60.15 | 165 | 83 |
100.00%
|
15.48%
|