| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 311 | Bacillus | TATTGCGCACGCTGCATG | AGCTTCGCGAAGTGCTTC | 59.60 | 58.44 | 193 | 83 |
100.00%
|
15.48%
|
| Primer 312 | Bacillus | ACTGGTACTGCAGAGCGTC | CGCCAACTTTCGTAGCAGC | 59.41 | 59.87 | 169 | 83 |
100.00%
|
15.48%
|
| Primer 313 | Bacillus | ACGCCAGAAATCGCATCAC | CGTTTTCACCGTAGCGTGG | 58.91 | 59.51 | 169 | 83 |
100.00%
|
15.48%
|
| Primer 314 | Bacillus | CGTAGCGGACGCAACAGTA | CCGTCGTAAATGCGATTTCCA | 60.15 | 59.34 | 174 | 83 |
100.00%
|
15.48%
|
| Primer 315 | Bacillus | CGTAGCGGACGCAACAGTA | TCCGTCGTAAATGCGATTTCC | 60.15 | 59.07 | 175 | 83 |
100.00%
|
15.48%
|
| Primer 316 | Bacillus | ATGGAAAGGGCACGCTAGC | CACAAACAGCTGCCGTCAC | 60.45 | 60.01 | 294 | 83 |
100.00%
|
15.48%
|
| Primer 317 | Bacillus | AAGGCGTTATCCTTGTGGCT | ACTTTGAAGCCTACGCGAGTA | 59.67 | 59.46 | 196 | 83 |
100.00%
|
15.48%
|
| Primer 318 | Bacillus | GCACTTCCAGAAGCAGGCT | GTGCCAAAGCGCTTGAACA | 60.30 | 59.93 | 177 | 83 |
100.00%
|
14.29%
|
| Primer 319 | Bacillus | GCACTTCCAGAAGCAGGCT | TGCCAAAGCGCTTGAACA | 60.30 | 58.48 | 176 | 83 |
100.00%
|
14.29%
|
| Primer 320 | Bacillus | TTTGCAGCAGGTGAAGGCT | ACGCCACCTGTTGCTTGA | 60.15 | 59.81 | 263 | 83 |
100.00%
|
15.48%
|