| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 431 | Bacillus | TTGCGCATGCTGGATGGA | ACTACTTGCTCTGCTGCTGT | 60.05 | 59.32 | 172 | 78 |
100.00%
|
14.29%
|
| Primer 432 | Bacillus | GAACAAAGACCCGTCCAACG | TCGATTGCTGCCCATCCA | 59.42 | 59.00 | 253 | 78 |
100.00%
|
15.48%
|
| Primer 433 | Bacillus | GAACAAAGACCCGTCCAACG | TGGATCGATTGCTGCCCA | 59.42 | 59.00 | 257 | 78 |
100.00%
|
15.48%
|
| Primer 434 | Bacillus | GAACAAAGACCCGTCCAACG | ATCGATTGCTGCCCATCCA | 59.42 | 59.47 | 254 | 78 |
100.00%
|
15.48%
|
| Primer 435 | Bacillus | AGCGGTGAAGTAGCACAAC | TCTCAATTGCAACCATGTCGA | 58.09 | 58.22 | 160 | 78 |
100.00%
|
13.10%
|
| Primer 436 | Bacillus | TGAAAGCGGTGAAGTAGCACA | TCTCAATTGCAACCATGTCGA | 60.20 | 58.22 | 164 | 78 |
100.00%
|
13.10%
|
| Primer 437 | Bacillus | AAGCGGTGAAGTAGCACAAC | TCTCAATTGCAACCATGTCGA | 58.77 | 58.22 | 161 | 78 |
100.00%
|
13.10%
|
| Primer 438 | Bacillus | CTGAAAGCGGTGAAGTAGCAC | TCTCAATTGCAACCATGTCGA | 59.54 | 58.22 | 165 | 78 |
100.00%
|
13.10%
|
| Primer 439 | Bacillus | GAAAGCGGTGAAGTAGCACA | TCTCAATTGCAACCATGTCGA | 58.49 | 58.22 | 163 | 78 |
100.00%
|
13.10%
|
| Primer 440 | Bacillus | TGAAAGCGGTGAAGTAGCAC | TCTCAATTGCAACCATGTCGA | 58.49 | 58.22 | 164 | 78 |
100.00%
|
13.10%
|