| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 451 | Bacillus | CGGGAGGACAAAAGCAACG | TTGCGCACGCTCACTTTTC | 59.43 | 60.01 | 287 | 78 |
100.00%
|
15.48%
|
| Primer 452 | Bacillus | CGGGAGGACAAAAGCAACG | AATTGTTGCGCACGCTCA | 59.43 | 58.97 | 292 | 78 |
100.00%
|
14.29%
|
| Primer 453 | Bacillus | CCGAAAGTAGCGGTGGAAAAC | CGACGAGATTCCTCATCCCC | 59.80 | 59.68 | 278 | 78 |
100.00%
|
15.48%
|
| Primer 454 | Bacillus | CCGAAAGTAGCGGTGGAAAAC | ACGACGAGATTCCTCATCCC | 59.80 | 58.97 | 279 | 78 |
100.00%
|
15.48%
|
| Primer 455 | Bacillus | TCTTGCTGTTGCTGGTCTCA | ACGTTTCCATCCGCAACC | 59.53 | 58.34 | 183 | 78 |
100.00%
|
15.48%
|
| Primer 456 | Bacillus | TCGAGCTCGATGTCCTTGC | AAACGGCTTGAAGCAGCG | 59.86 | 59.36 | 268 | 78 |
100.00%
|
14.29%
|
| Primer 457 | Bacillus | GGGGAAGTTTCACGCGTTG | AGCTTTGTGACGTGTGCG | 59.72 | 58.99 | 191 | 78 |
100.00%
|
14.29%
|
| Primer 458 | Bacillus | CCCAGAACGTAGTGAGGGGA | ACACGCTGCATTTGCTCC | 60.61 | 59.04 | 255 | 78 |
100.00%
|
15.48%
|
| Primer 459 | Bacillus | TGGTGTTGGTGATGCGGT | ACTTTCGCACCGATTGGC | 59.49 | 58.74 | 153 | 78 |
100.00%
|
15.48%
|
| Primer 460 | Bacillus | TGCTCGTGGGCATCATTTG | CGCACTTTGACAACCGTCC | 58.82 | 59.72 | 189 | 78 |
100.00%
|
15.48%
|