| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 471 | Bacillus | ACTGCAATCGGCTTCGGT | GCCAACCTTTCCCTTGTGC | 59.65 | 59.63 | 190 | 78 |
100.00%
|
13.10%
|
| Primer 472 | Bacillus | TCGATTCGAGTGCGTACCA | TCTGCATTCCCTTGCGCA | 58.83 | 59.97 | 198 | 78 |
100.00%
|
15.48%
|
| Primer 473 | Bacillus | AGTGGACATGCACCTGAGC | CTCGTACTTCGCCTGTTCCA | 60.00 | 59.76 | 163 | 78 |
100.00%
|
14.29%
|
| Primer 474 | Bacillus | AAGGCGTTATCCTTGTGGC | ACTTTGAAGCCTACGCGAGTA | 58.13 | 59.46 | 196 | 78 |
100.00%
|
15.48%
|
| Primer 475 | Bacillus | TCCCAAAGCAAAACCGTGT | ACTTTGAAGCCTACGCGAGTA | 58.11 | 59.46 | 175 | 78 |
100.00%
|
15.48%
|
| Primer 476 | Bacillus | TGCAGCGAAAGAGGCGTA | AGCGTGACGAGCTTTCTAAAA | 59.35 | 58.25 | 197 | 78 |
100.00%
|
15.48%
|
| Primer 477 | Bacillus | TGCAGCGAAAGAGGCGTA | GCGTGACGAGCTTTCTAAAAC | 59.35 | 58.18 | 196 | 78 |
100.00%
|
15.48%
|
| Primer 478 | Bacillus | TTGCAGCGAAAGAGGCGTA | AGCGTGACGAGCTTTCTAAAA | 60.01 | 58.25 | 198 | 78 |
100.00%
|
15.48%
|
| Primer 479 | Bacillus | TTGCAGCGAAAGAGGCGTA | GCGTGACGAGCTTTCTAAAAC | 60.01 | 58.18 | 197 | 78 |
100.00%
|
15.48%
|
| Primer 480 | Bacillus | TCCGATTCGCGTATCTCGATC | TTCAACCCACTGCTCGTCC | 59.87 | 59.93 | 268 | 78 |
100.00%
|
13.10%
|