| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 491 | Bacillus | TGGCGATGGTTCAGAAGGA | TGCCTTCACCCAATTCCCT | 58.63 | 58.83 | 215 | 77 |
100.00%
|
16.67%
|
| Primer 492 | Bacillus | GGCATGCATGGGTTGTTGG | AGGCCACCTACCTCTTCCT | 60.08 | 59.21 | 217 | 77 |
100.00%
|
16.67%
|
| Primer 493 | Bacillus | TCAGAGGAAGCACAAGGGTT | CCGCATAAAAGCCTTAGCGC | 58.86 | 60.32 | 192 | 77 |
100.00%
|
16.67%
|
| Primer 494 | Bacillus | TGCAAGCATGGCAACGTT | AGAAGTGCATCCCCTTCTTGT | 58.87 | 59.29 | 151 | 77 |
100.00%
|
13.10%
|
| Primer 495 | Bacillus | CGTGAGCATGGAAGCGGTA | AGCACGCCAGCAGAAGTT | 60.15 | 59.89 | 242 | 77 |
100.00%
|
14.29%
|
| Primer 496 | Bacillus | TGGCTCGATTCCATTCGCA | AGCACGCCAGCAGAAGTT | 59.78 | 59.89 | 297 | 77 |
100.00%
|
14.29%
|
| Primer 497 | Bacillus | GGCTCGATTCCATTCGCAC | AGCACGCCAGCAGAAGTT | 59.36 | 59.89 | 296 | 77 |
100.00%
|
14.29%
|
| Primer 498 | Bacillus | TTCGTGAGCATGGAAGCGG | AGCACGCCAGCAGAAGTT | 60.74 | 59.89 | 244 | 77 |
100.00%
|
14.29%
|
| Primer 499 | Bacillus | AAAGCAACGTCGGTCCCT | GCTGTACGCTCTTCCCCAT | 59.17 | 59.48 | 215 | 77 |
100.00%
|
15.48%
|
| Primer 500 | Bacillus | TGCAAACGCCATATGACCCT | ACACGTGCCCATTCCCAAT | 60.03 | 59.92 | 166 | 77 |
100.00%
|
15.48%
|