| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 521 | Bacillus | GCTGATCCAAGTGAAGGGCA | AGCAGTTTCTACCGCATGC | 60.32 | 58.53 | 204 | 76 |
100.00%
|
13.10%
|
| Primer 522 | Bacillus | AAATGGCGTGGGCTCACT | AACCACCGCTTATCGCAGC | 59.56 | 61.11 | 271 | 76 |
100.00%
|
13.10%
|
| Primer 523 | Bacillus | AAATGGCGTGGGCTCACT | TCGCAGCTACTACTCCTGC | 59.56 | 58.89 | 259 | 76 |
100.00%
|
14.29%
|
| Primer 524 | Bacillus | AAATGGCGTGGGCTCACT | ACCGCTTATCGCAGCTACT | 59.56 | 58.88 | 267 | 76 |
100.00%
|
13.10%
|
| Primer 525 | Bacillus | AAATGGCGTGGGCTCACT | CACCGCTTATCGCAGCTACT | 59.56 | 60.25 | 268 | 76 |
100.00%
|
13.10%
|
| Primer 526 | Bacillus | ACGGGAAGCGTTAAAGAATGC | TCCTGTAACGGCTGCAACT | 59.53 | 59.25 | 252 | 76 |
100.00%
|
13.10%
|
| Primer 527 | Bacillus | ACCGTTGTGAGCCAGTTGG | CCGCTAATTCACGAGCTGC | 60.53 | 59.36 | 180 | 76 |
100.00%
|
15.48%
|
| Primer 528 | Bacillus | TCAGCAACGAACGCCTCA | GGATACCATCCCCACGCAA | 59.58 | 59.47 | 233 | 76 |
100.00%
|
14.29%
|
| Primer 529 | Bacillus | CGTGAGCATGGAAGCGGTA | AGCACCCCAGCAGAAGTT | 60.15 | 58.74 | 242 | 76 |
100.00%
|
14.29%
|
| Primer 530 | Bacillus | GAACAAAGACCCGTCCAACG | TCGATTGCTGCCCATCCAA | 59.42 | 59.70 | 253 | 76 |
100.00%
|
15.48%
|