| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 531 | Bacillus | GCGACAGTGTTTGGTGGTC | CAAATGCTGCCCACGCAA | 59.35 | 59.66 | 175 | 76 |
100.00%
|
15.48%
|
| Primer 532 | Bacillus | CATTCCCAGGTCAAGGGCA | ACTTGTCCGTATGCTGGGC | 59.62 | 60.08 | 160 | 76 |
100.00%
|
15.48%
|
| Primer 533 | Bacillus | TTCCCAGGTCAAGGGCATG | TTGTCCGTATGCTGGGCTC | 59.62 | 59.78 | 156 | 76 |
100.00%
|
15.48%
|
| Primer 534 | Bacillus | TCACCAATGGGCTGCCAA | GCTTCGCTTGACCACATTGT | 59.47 | 59.41 | 154 | 76 |
92.31%
|
14.29%
|
| Primer 535 | Bacillus | TGGGCAAAGCACACGGTT | AGCAACGCGTGGGTCAAT | 60.44 | 60.28 | 254 | 76 |
100.00%
|
13.10%
|
| Primer 536 | Bacillus | TGGGCAAAGCACACGGTT | ACATAGCAACGCGTGGGT | 60.44 | 59.65 | 258 | 76 |
100.00%
|
13.10%
|
| Primer 537 | Bacillus | TGGGCAAAGCACACGGTT | TAGCAACGCGTGGGTCAA | 60.44 | 59.58 | 255 | 76 |
100.00%
|
13.10%
|
| Primer 538 | Bacillus | AGCAGCTAGTATCGGCTTAGC | GGACTCCCGAGCTTTGACT | 59.73 | 59.02 | 153 | 76 |
100.00%
|
14.29%
|
| Primer 539 | Bacillus | TCCCAGGTAAAGGGCATGC | TTGTCCGTATGCTGGGCTC | 59.69 | 59.78 | 155 | 76 |
100.00%
|
14.29%
|
| Primer 540 | Bacillus | GGTATGTGACAGGACCGCA | GCAGACTCTTTGCTTGTCCC | 59.41 | 59.12 | 184 | 76 |
100.00%
|
14.29%
|