| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 51 | Bacillus | GCAAGCAACCGATGACACC | TCAAACGGGAGATGGCGTG | 59.79 | 60.38 | 238 | 104 |
100.00%
|
15.48%
|
| Primer 52 | Bacillus | TATCGCAGAGAGCATCGCG | GCACCTTTACCGGAACCCA | 60.08 | 59.93 | 242 | 104 |
100.00%
|
23.81%
|
| Primer 53 | Bacillus | ATGGCTCGCAGCTTGAAAA | ACCCAACTTGTGCCCTACC | 58.36 | 59.54 | 216 | 104 |
100.00%
|
14.29%
|
| Primer 54 | Bacillus | TCTGAGCAAGGAGGCGTT | TGTCCGTTAAGCGCTGCT | 58.53 | 59.66 | 217 | 104 |
100.00%
|
15.48%
|
| Primer 55 | Bacillus | TTCTGAGCAAGGAGGCGTT | TGTCCGTTAAGCGCTGCT | 59.25 | 59.66 | 218 | 104 |
100.00%
|
15.48%
|
| Primer 56 | Bacillus | AATCGCTGCTGCCCTTGT | AGCCATGACGAAGCACCT | 59.97 | 58.93 | 216 | 104 |
100.00%
|
14.29%
|
| Primer 57 | Bacillus | CCCCACAGCAGATGGTTCA | TGCAACTTCAGCGATTCGTT | 59.62 | 58.77 | 251 | 104 |
100.00%
|
14.29%
|
| Primer 58 | Bacillus | GCTGGCGAGCAAGGATTTG | TGCACGATCCATGCCCAT | 59.86 | 59.40 | 159 | 103 |
100.00%
|
14.29%
|
| Primer 59 | Bacillus | AGGTGGTACGACACTTGCTC | CATTGCTTCTTGCGCTTGC | 59.68 | 58.93 | 184 | 103 |
100.00%
|
15.48%
|
| Primer 60 | Bacillus | ATGGCTCGCAGCTTGAAAA | CCCAACTTGTGCCCTACCA | 58.36 | 59.54 | 215 | 102 |
92.31%
|
14.29%
|