| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Bacillus | TGGTACGACACTTGCTCGTG | CATTGCTTCTTGCGCTTGC | 60.32 | 58.93 | 181 | 111 |
100.00%
|
15.48%
|
| Primer 32 | Bacillus | GGTACGACACTTGCTCGTG | CATTGCTTCTTGCGCTTGC | 58.57 | 58.93 | 180 | 111 |
100.00%
|
15.48%
|
| Primer 33 | Bacillus | TCTGAGCAAGGAGGCGTTC | TGTCCGTTAAGCGCTGCT | 59.71 | 59.66 | 217 | 110 |
100.00%
|
15.48%
|
| Primer 34 | Bacillus | TGGTTCTGAGCAAGGAGGC | TGTCCGTTAAGCGCTGCT | 59.62 | 59.66 | 221 | 110 |
100.00%
|
15.48%
|
| Primer 35 | Bacillus | CGCCAGAAATCGCATCACA | CGTTTTCACCGTAGCGTGG | 58.91 | 59.51 | 168 | 110 |
100.00%
|
15.48%
|
| Primer 36 | Bacillus | CGGTGATGCGTTAGAAACAGG | TGTCGTTACAAGGCCATGGT | 59.61 | 59.60 | 175 | 110 |
100.00%
|
14.29%
|
| Primer 37 | Bacillus | TGACGCACATGACGAGCA | ATTGCGATGCCGTGACCA | 59.66 | 60.05 | 249 | 108 |
100.00%
|
15.48%
|
| Primer 38 | Bacillus | AGGACGCGATATGACTGAACT | TGTCCCATGAGTCCAACTAGC | 58.97 | 59.44 | 199 | 107 |
100.00%
|
15.48%
|
| Primer 39 | Bacillus | AGGACGCGATATGACTGAACT | CACCTTCTTGTAAGCCACCTC | 58.97 | 58.57 | 176 | 107 |
100.00%
|
15.48%
|
| Primer 40 | Bacillus | ACAAAGAGTTATGGCATGCGC | ACCCCTGGAAACTTTCGCC | 59.87 | 60.23 | 181 | 107 |
100.00%
|
15.48%
|