| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Bacillus | TGCCCAATTGCAACGACAC | CCATCAGCTTCAAGTTGCCG | 59.64 | 59.83 | 164 | 117 |
100.00%
|
15.48%
|
| Primer 12 | Bacillus | TGCAGCGACGAAACATGC | TTCGGACCAATGCCCATCC | 59.44 | 60.08 | 261 | 114 |
100.00%
|
14.29%
|
| Primer 13 | Bacillus | AGCAATTTCGCAGCTTGAGC | GCTCTCCATCTTCCCCAAGG | 60.11 | 59.82 | 170 | 114 |
100.00%
|
15.48%
|
| Primer 14 | Bacillus | GCAATTTCGCAGCTTGAGC | GCTCTCCATCTTCCCCAAGG | 58.64 | 59.82 | 169 | 114 |
100.00%
|
15.48%
|
| Primer 15 | Bacillus | ACAGCGGTTCGATTGGCA | TCGCACCACGTAACACATGA | 59.97 | 59.97 | 262 | 114 |
100.00%
|
15.48%
|
| Primer 16 | Bacillus | CGGGTGAAGGGCTTGTTGA | TGAGCAGCTTGGCGAACA | 60.23 | 59.89 | 220 | 114 |
100.00%
|
15.48%
|
| Primer 17 | Bacillus | CCCCACAGCAGATGGTTCA | AGCGATTCGTTCATAGCGACT | 59.62 | 59.93 | 242 | 114 |
100.00%
|
14.29%
|
| Primer 18 | Bacillus | CCCCACAGCAGATGGTTCA | CAGCGATTCGTTCATAGCGAC | 59.62 | 59.81 | 243 | 114 |
100.00%
|
14.29%
|
| Primer 19 | Bacillus | CCCCACAGCAGATGGTTCA | GCAACTTCAGCGATTCGTTCA | 59.62 | 59.81 | 250 | 114 |
100.00%
|
14.29%
|
| Primer 20 | Bacillus | CCCCACAGCAGATGGTTCA | TGCAACTTCAGCGATTCGTTC | 59.62 | 59.81 | 251 | 114 |
100.00%
|
14.29%
|