| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Bacillus | ACGCCAGAAATCGCATCAC | CGTTTTCACCGTAGCGTGG | 58.91 | 59.51 | 169 | 142 |
100.00%
|
15.48%
|
| Primer 2 | Bacillus | CGCCAGAAATCGCATCACAC | CGTTTTCACCGTAGCGTGG | 60.25 | 59.51 | 168 | 133 |
100.00%
|
15.48%
|
| Primer 3 | Bacillus | AACGCCAGAAATCGCATCAC | CGTTTTCACCGTAGCGTGG | 59.55 | 59.51 | 170 | 133 |
100.00%
|
15.48%
|
| Primer 4 | Bacillus | TGCAGCGACGAAACATGC | TTCGGACCAATGCCCATCC | 59.44 | 60.08 | 261 | 123 |
100.00%
|
14.29%
|
| Primer 5 | Bacillus | CGTAGCACTTGAAACTGCAGG | TGTGCGTTTGAAATACGGCTG | 59.80 | 60.07 | 234 | 121 |
100.00%
|
15.48%
|
| Primer 6 | Bacillus | GCAAGCAACCGATGACACC | TCAAACGGGAGATGGCGTG | 59.79 | 60.38 | 238 | 120 |
100.00%
|
15.48%
|
| Primer 7 | Bacillus | ACAGCGGTTCGATTGGCA | CCGGTCCAATTCCCTCCAT | 59.97 | 59.08 | 289 | 120 |
100.00%
|
15.48%
|
| Primer 8 | Bacillus | ACAGCGGTTCGATTGGCA | TTCCGGTCCAATTCCCTCC | 59.97 | 59.00 | 291 | 120 |
100.00%
|
15.48%
|
| Primer 9 | Bacillus | ACAGCGGTTCGATTGGCA | TCCGGTCCAATTCCCTCCA | 59.97 | 60.23 | 290 | 117 |
100.00%
|
15.48%
|
| Primer 10 | Bacillus | ACGCCAGGACATTCTCCAG | TGTCCACATAGCACCGCTG | 59.40 | 60.08 | 191 | 117 |
100.00%
|
15.48%
|