| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 601 | Bacillus | TCCAGATTGCCGTGACGT | TGCTGGACATTCACCGCA | 58.94 | 59.57 | 214 | 74 |
100.00%
|
15.48%
|
| Primer 602 | Bacillus | TCGGCAGCGAAAGAAATGAA | TGTCCATTCTTGCAAACGACC | 58.48 | 59.39 | 165 | 74 |
100.00%
|
16.67%
|
| Primer 603 | Bacillus | TGCTGATCCAAGTGAAGGGC | AGCAGTTTCTACCGCATGC | 60.32 | 58.53 | 205 | 74 |
100.00%
|
13.10%
|
| Primer 604 | Bacillus | TGCAAGCATGGCAACGTT | ATGTCTTTTGCTTCACGCGT | 58.87 | 59.06 | 298 | 74 |
100.00%
|
14.29%
|
| Primer 605 | Bacillus | TGCAAGCATGGCAACGTT | AATGTCTTTTGCTTCACGCGT | 58.87 | 59.67 | 299 | 74 |
100.00%
|
13.10%
|
| Primer 606 | Bacillus | CCGCTTGGTGTGAAAACAAGT | TGAAGCAGCCCACACCAT | 59.87 | 59.15 | 234 | 74 |
100.00%
|
15.48%
|
| Primer 607 | Bacillus | AGAAGGAAATCGCAGGGGAA | TCACGCACAACCTCAGCA | 59.01 | 59.50 | 259 | 74 |
100.00%
|
13.10%
|
| Primer 608 | Bacillus | GAGAAGGAAATCGCAGGGGAA | TCACGCACAACCTCAGCA | 60.07 | 59.50 | 260 | 74 |
100.00%
|
13.10%
|
| Primer 609 | Bacillus | TATTGCGCACGCTGCATG | ACAGCCAGCTTCGCGAA | 59.60 | 59.60 | 199 | 74 |
100.00%
|
15.48%
|
| Primer 610 | Bacillus | CGGGAGGACAAAAGCAACG | GTTGCGCACGCTCACTTT | 59.43 | 59.67 | 288 | 74 |
100.00%
|
14.29%
|