| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 621 | Bacillus | AGCATGGATCACGCTTGGT | CCATAGCTTGGCGATACTCCA | 59.70 | 59.66 | 187 | 74 |
100.00%
|
13.10%
|
| Primer 622 | Bacillus | ACGCCAGAAATCGCATCACA | CGTTTTCACCGTAGCGTGG | 60.67 | 59.51 | 169 | 74 |
100.00%
|
15.48%
|
| Primer 623 | Bacillus | ACCGCTTGGATTTTCAACGG | GCTGTATGAGCTTTCTCGCC | 59.40 | 59.07 | 165 | 74 |
100.00%
|
15.48%
|
| Primer 624 | Bacillus | GCTGGCGAGCAAGGATTTG | TGCACGATCCATGCCCAT | 59.86 | 59.40 | 159 | 74 |
100.00%
|
14.29%
|
| Primer 625 | Bacillus | GGGCAAGCTCAAAGAACAGC | ACTCGCTGCATACGGATCAT | 60.04 | 59.32 | 186 | 74 |
100.00%
|
15.48%
|
| Primer 626 | Bacillus | GTATTGCGCACGCTGCAT | CAGCTTCGCGAAGTGCTTC | 59.60 | 59.87 | 195 | 74 |
100.00%
|
15.48%
|
| Primer 627 | Bacillus | GCGAACTTGCAAGAAGAAGCA | CCGCTCACTGGTGGGATTAG | 60.00 | 60.18 | 206 | 74 |
100.00%
|
14.29%
|
| Primer 628 | Bacillus | TGCGAACTTGCAAGAAGAAGC | CCGCTCACTGGTGGGATTAG | 60.00 | 60.18 | 207 | 74 |
100.00%
|
14.29%
|
| Primer 629 | Bacillus | TCGAGCTCGATGTCCTTGC | AAACGGCTTGAAGCAGCG | 59.86 | 59.36 | 268 | 74 |
100.00%
|
14.29%
|
| Primer 630 | Bacillus | CGAGCTCGATGTCCTTGCT | AAACGGCTTGAAGCAGCG | 59.86 | 59.36 | 267 | 74 |
100.00%
|
14.29%
|