| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 171 | Brachyspira | CGGATGCGGTTCTTCAAGAG | ACTCAGGAAAAGGCTCTGCT | 59.00 | 58.94 | 248 | 84 |
100.00%
|
100.00%
|
| Primer 172 | Brachyspira | TGCGGTTCTTCAAGAGAGCAT | ACTCAGGAAAAGGCTCTGCT | 60.00 | 58.94 | 244 | 84 |
100.00%
|
100.00%
|
| Primer 173 | Brachyspira | ATGCGGTTCTTCAAGAGAGCA | ACTCAGGAAAAGGCTCTGCT | 60.00 | 58.94 | 245 | 84 |
100.00%
|
100.00%
|
| Primer 174 | Brachyspira | GATGCTCCTGGACTTGGCT | GCCGTAGCTTCAGCATGGA | 59.39 | 60.15 | 185 | 84 |
100.00%
|
50.00%
|
| Primer 175 | Brachyspira | AGGCTGCGGAAATGGAAAAT | TGGCCAAGGATCTGGAAAGT | 58.44 | 58.92 | 235 | 84 |
100.00%
|
50.00%
|
| Primer 176 | Brachyspira | GAAATAGGCTGCGGAAATGGA | TTGGCCAAGGATCTGGAAAGT | 58.70 | 59.57 | 241 | 84 |
100.00%
|
50.00%
|
| Primer 177 | Brachyspira | TGCCAGCGGAGAATTAGCT | TCCTCCTCTTCCGGATCTGC | 59.09 | 60.76 | 231 | 84 |
100.00%
|
50.00%
|
| Primer 178 | Brachyspira | TGCCAGCGGAGAATTAGCT | CTCCTCTTCCGGATCTGCATC | 59.09 | 60.00 | 229 | 84 |
100.00%
|
50.00%
|
| Primer 179 | Brachyspira | AGGTGCTAGAGTATCAGGACC | ACATCAACCCCAGCTGGA | 58.05 | 58.08 | 198 | 84 |
100.00%
|
50.00%
|
| Primer 180 | Brachyspira | AGGTGCTAGAGTATCAGGACC | GAACATCAACCCCAGCTGGA | 58.05 | 59.96 | 200 | 84 |
100.00%
|
50.00%
|