| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 191 | Brachyspira | TGGGAGAGATAATTGGAGGCA | CACCAATCCCCAGGTCCATAG | 58.22 | 59.86 | 265 | 84 |
100.00%
|
50.00%
|
| Primer 192 | Brachyspira | TGGGAGAGATAATTGGAGGCA | ATACCACCAATCCCCAGGTC | 58.22 | 58.78 | 269 | 84 |
100.00%
|
50.00%
|
| Primer 193 | Brachyspira | GCCACAAACACAAACGGCA | CAGCAGTGAGAAGGGCTGT | 60.15 | 59.63 | 232 | 84 |
100.00%
|
50.00%
|
| Primer 194 | Brachyspira | GCCTGTTGATGTAAGACCGGA | AGTGTGCGAACGCTTTGT | 60.07 | 58.19 | 200 | 83 |
100.00%
|
50.00%
|
| Primer 195 | Brachyspira | ACGGCAGTCAAGGACATGC | TCAGCAGCAACCTCATCTGG | 60.67 | 60.04 | 190 | 83 |
100.00%
|
75.00%
|
| Primer 196 | Brachyspira | ACCCAAGAACTTGCCCTGT | GCGGACAAACAGCTACAACA | 59.07 | 59.06 | 171 | 83 |
100.00%
|
50.00%
|
| Primer 197 | Brachyspira | AGAAGCTGCGGAGAACACC | AGCACAGCCAAAGCCAAAA | 60.00 | 58.79 | 185 | 83 |
100.00%
|
50.00%
|
| Primer 198 | Brachyspira | CCTGCCATGGGAGCTGAAA | TGGCGTCCTCAACCCAATC | 60.00 | 60.00 | 151 | 83 |
100.00%
|
50.00%
|
| Primer 199 | Brachyspira | CGGAGAAGGAGAATGCGGA | CCGGAGTACAGAAACCGCA | 59.18 | 59.71 | 194 | 82 |
100.00%
|
50.00%
|
| Primer 200 | Brachyspira | CACCCGATTTGCTTCCTGC | TCTGTCAACCTGTGCCTCAG | 59.49 | 59.61 | 287 | 82 |
100.00%
|
50.00%
|