| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 301 | Brachyspira | TGCCTAGTTTTGCAAACCCG | AGCAACAGGGTCATCTGTAGC | 59.33 | 60.07 | 152 | 73 |
100.00%
|
75.00%
|
| Primer 302 | Brachyspira | TGCCTAGTTTTGCAAACCCG | GCAACAGGGTCATCTGTAGC | 59.33 | 58.63 | 151 | 73 |
100.00%
|
75.00%
|
| Primer 303 | Brachyspira | TGCCTAGTTTTGCAAACCCG | GCCTTAGCAACAGGGTCATC | 59.33 | 58.62 | 157 | 73 |
100.00%
|
75.00%
|
| Primer 304 | Brachyspira | TTGCACACTTTATGACGGCA | GCTCCTCCCAAAATAGCTCCA | 58.40 | 59.79 | 168 | 73 |
100.00%
|
50.00%
|
| Primer 305 | Brachyspira | TGCACACTTTATGACGGCAT | GCTCCTCCCAAAATAGCTCCA | 58.18 | 59.79 | 167 | 73 |
100.00%
|
50.00%
|
| Primer 306 | Brachyspira | ATGGCTGGGAGAAGATGGC | GCACTTTGCGGAAATGTACCA | 59.46 | 59.73 | 256 | 73 |
100.00%
|
75.00%
|
| Primer 307 | Brachyspira | CCTGCCATGGGAGCTGAAA | TTTTGGCGTCCTCAACCCA | 60.00 | 59.77 | 154 | 73 |
100.00%
|
50.00%
|
| Primer 308 | Brachyspira | GGCTACGGACTTAAAGAGGCT | ATCATTAGTGGCGTCGTACCA | 59.52 | 59.25 | 252 | 72 |
100.00%
|
50.00%
|
| Primer 309 | Brachyspira | GGCTACGGACTTAAAGAGGCT | CATTAGTGGCGTCGTACCAA | 59.52 | 58.00 | 250 | 72 |
100.00%
|
50.00%
|
| Primer 310 | Brachyspira | GGTGCTTCAGGCTCTGGAA | TCTGCTTCATTCCTCCGCT | 59.62 | 58.71 | 259 | 72 |
100.00%
|
50.00%
|