| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 311 | Brachyspira | AGGTGCTTCAGGCTCTGGA | TCTGCTTCATTCCTCCGCT | 60.54 | 58.71 | 260 | 72 |
100.00%
|
50.00%
|
| Primer 312 | Brachyspira | AGGCTGCGGAAATGGAAAAT | TTGGCCAAGGATCTGGAAAGT | 58.44 | 59.57 | 236 | 72 |
100.00%
|
50.00%
|
| Primer 313 | Brachyspira | AGTTGGCAAACCTTTAGCTCA | ACACTTCCAACCCCATTAGGT | 58.06 | 58.93 | 288 | 72 |
100.00%
|
50.00%
|
| Primer 314 | Brachyspira | AGCATATTCCAAGGTGCTGCT | AGCACCTAAAACACCGCTGA | 60.06 | 59.89 | 222 | 72 |
100.00%
|
50.00%
|
| Primer 315 | Brachyspira | TCCGCAAAGAGAAGGGGT | ACACCTGTAGCTTCACGGC | 58.10 | 60.00 | 240 | 72 |
100.00%
|
75.00%
|
| Primer 316 | Brachyspira | TGCTGGCGAATCTATGGCT | ACCTTCCCAAGCAGCTTGT | 59.17 | 59.46 | 190 | 72 |
100.00%
|
100.00%
|
| Primer 317 | Brachyspira | TTTTGCAGTCGCTTGAGCT | GGGCTTCGCTTACCAATGC | 58.29 | 59.57 | 219 | 72 |
100.00%
|
75.00%
|
| Primer 318 | Brachyspira | AACAAGCGGAGGAGATGCT | TGATGCCTCCTACGCTTCC | 59.01 | 59.18 | 161 | 72 |
100.00%
|
50.00%
|
| Primer 319 | Brachyspira | CCTATAGGCTGCCGTGTAACT | GCACGGAATGGCAAACCT | 59.31 | 58.63 | 287 | 72 |
100.00%
|
50.00%
|
| Primer 320 | Brachyspira | CCAAGCAGATTCGGTGTTGC | GCAGTCATACCAGCCGTCTA | 60.11 | 59.26 | 264 | 72 |
100.00%
|
50.00%
|