| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 441 | Brachyspira | ATTGCACACTTTATGACGGCA | GCTCCTCCCAAAATAGCTCCA | 58.84 | 59.79 | 169 | 66 |
100.00%
|
50.00%
|
| Primer 442 | Brachyspira | GCCCTCCCCTCCTGGTAAAT | TCCGGAAAGTTCATGCGGAT | 60.99 | 59.75 | 298 | 66 |
100.00%
|
50.00%
|
| Primer 443 | Brachyspira | GCCCTCCCCTCCTGGTAAAT | CTCCGGAAAGTTCATGCGGA | 60.99 | 60.39 | 299 | 66 |
100.00%
|
50.00%
|
| Primer 444 | Brachyspira | GCCCTCCCCTCCTGGTAAAT | CCGGAAAGTTCATGCGGAT | 60.99 | 58.22 | 297 | 66 |
100.00%
|
50.00%
|
| Primer 445 | Brachyspira | TCGCTTTAGGCTGTCCTGC | TTTGGCGTCCTCAACCCA | 60.08 | 59.08 | 167 | 66 |
100.00%
|
50.00%
|
| Primer 446 | Brachyspira | GCCAGCGGAGAATTAGCTCA | CTCCTCTTCCGGATCTGCA | 60.18 | 58.49 | 228 | 65 |
100.00%
|
50.00%
|
| Primer 447 | Brachyspira | GCCAGCGGAGAATTAGCTCA | CCTCTTCCGGATCTGCATCA | 60.18 | 59.24 | 226 | 65 |
100.00%
|
50.00%
|
| Primer 448 | Brachyspira | TGCCAGCGGAGAATTAGCTC | CCTCTTCCGGATCTGCATCA | 60.18 | 59.24 | 227 | 65 |
100.00%
|
50.00%
|
| Primer 449 | Brachyspira | GGTGCTGGTATTGCTGTTGG | GCACCTAAAACACCGCTGA | 59.47 | 58.38 | 194 | 65 |
100.00%
|
100.00%
|
| Primer 450 | Brachyspira | CGCTTTGATGGTTTTAGGCGA | ATACAGCACGAGCAGGACC | 59.53 | 59.48 | 242 | 65 |
100.00%
|
50.00%
|