| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 451 | Brachyspira | TTGCCTGTGCTGAAAAGCA | AACATCGGCACCAGCTTCA | 58.50 | 59.93 | 161 | 65 |
100.00%
|
50.00%
|
| Primer 452 | Brachyspira | AGCGGAAGTGCTGTTGCA | CCTGCCATCTTTCCTGCCT | 60.20 | 59.69 | 184 | 65 |
100.00%
|
50.00%
|
| Primer 453 | Brachyspira | GGTCCTGCACGTGCTGTAT | GCCCTCCCATAAATCTGGTCA | 60.08 | 59.51 | 179 | 65 |
100.00%
|
100.00%
|
| Primer 454 | Brachyspira | GGTCCTGCACGTGCTGTAT | CAGCCCTCCCATAAATCTGGT | 60.08 | 59.50 | 181 | 65 |
100.00%
|
100.00%
|
| Primer 455 | Brachyspira | GCTTGCCCTGCTTCTCCAA | GCAGCTTCTCACTCCGTCT | 60.60 | 59.41 | 213 | 65 |
100.00%
|
50.00%
|
| Primer 456 | Brachyspira | TTGCTTGCCCTGCTTCTCC | GCAGCTTCTCACTCCGTCT | 60.60 | 59.41 | 215 | 65 |
100.00%
|
50.00%
|
| Primer 457 | Brachyspira | TGGGAGAGATAATTGGAGGCA | CACCAATCCCCAGGTCCATA | 58.22 | 58.78 | 265 | 65 |
100.00%
|
50.00%
|
| Primer 458 | Brachyspira | TGCCTAGTTTTGCAAACCCG | GCCTTAGCAACAGGGTCATCT | 59.33 | 60.07 | 157 | 65 |
100.00%
|
75.00%
|
| Primer 459 | Brachyspira | AGACGGAAGCGGTAAGTCT | CCCTTAGCACCTTGATAGGCA | 58.04 | 59.51 | 285 | 65 |
100.00%
|
50.00%
|
| Primer 460 | Brachyspira | AGACGGAAGCGGTAAGTCT | ACCCTTAGCACCTTGATAGGC | 58.04 | 59.51 | 286 | 65 |
100.00%
|
50.00%
|