| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 541 | Brachyspira | CTCCTGCTCTTGGAGTGGTA | ACATCTTCAGGCTGCGGT | 58.44 | 58.93 | 199 | 59 |
100.00%
|
50.00%
|
| Primer 542 | Brachyspira | AATCTGCTCCTGCTCTTGGA | AACATCTTCAGGCTGCGGT | 58.72 | 59.62 | 206 | 59 |
100.00%
|
50.00%
|
| Primer 543 | Brachyspira | TGCGAGATAGCAAGGGCA | ACGTGCCAAAGGATTCGCT | 58.69 | 60.30 | 258 | 59 |
100.00%
|
50.00%
|
| Primer 544 | Brachyspira | AAACAAACCGCTCTTCGCT | AACCATGAAAAGGGCATGCT | 58.30 | 58.35 | 161 | 59 |
100.00%
|
50.00%
|
| Primer 545 | Brachyspira | AAACAAACCGCTCTTCGCT | AAACCATGAAAAGGGCATGCT | 58.30 | 59.02 | 162 | 59 |
100.00%
|
50.00%
|
| Primer 546 | Brachyspira | AGATGAGCCCACTAATGGTGT | TCCATCTGGATGCTTGTGCT | 58.80 | 59.38 | 229 | 59 |
100.00%
|
50.00%
|
| Primer 547 | Brachyspira | TCGCTTTAGGCTGTCCTGC | TGGCGTCCTCAACCCAAT | 60.08 | 58.83 | 165 | 59 |
100.00%
|
50.00%
|
| Primer 548 | Brachyspira | GGTGCTTCAGGCTCTGGAAA | TCTCTGCTTCATTCCTCCGC | 60.25 | 59.82 | 261 | 58 |
100.00%
|
50.00%
|
| Primer 549 | Brachyspira | TGCGGTTCTTCAAGAGAGCA | ACTCAGGAAAAGGCTCTGCTT | 59.61 | 59.58 | 244 | 58 |
100.00%
|
100.00%
|
| Primer 550 | Brachyspira | TCGGATGCGGTTCTTCAAGA | ACTCAGGAAAAGGCTCTGCTT | 59.39 | 59.58 | 249 | 58 |
100.00%
|
100.00%
|