| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 551 | Brachyspira | TCGGATGCGGTTCTTCAAGA | AACTCAGGAAAAGGCTCTGCT | 59.39 | 59.58 | 250 | 58 |
100.00%
|
100.00%
|
| Primer 552 | Brachyspira | TGCTGGCGAATCTATGGCT | TCACCTTCCCAAGCAGCTT | 59.17 | 59.15 | 192 | 58 |
100.00%
|
100.00%
|
| Primer 553 | Brachyspira | ATCAGCATGTTTGAACGCCT | GACGCCTCCTTAGCTCTGT | 58.46 | 58.81 | 242 | 58 |
100.00%
|
50.00%
|
| Primer 554 | Brachyspira | TCAGCATGTTTGAACGCCTT | GACGCCTCCTTAGCTCTGTT | 58.68 | 59.47 | 241 | 58 |
100.00%
|
50.00%
|
| Primer 555 | Brachyspira | GCCTGTTGATGTAAGACCGGA | AGTGTGCGAACGCTTTGTT | 60.07 | 58.91 | 200 | 58 |
100.00%
|
50.00%
|
| Primer 556 | Brachyspira | TCTTGGGCTAGTGCAGGTT | TCCGGAAGCTTCAACTGCT | 58.54 | 59.25 | 189 | 58 |
100.00%
|
50.00%
|
| Primer 557 | Brachyspira | AGCGATGACAGCTGGGTT | TGCTTGCTGTAGGATACCCC | 58.93 | 59.16 | 151 | 58 |
100.00%
|
75.00%
|
| Primer 558 | Brachyspira | GGCCTCGTCATTGTGTTGC | TTGAGCTGTATCCCTTGCCG | 59.79 | 60.11 | 242 | 58 |
100.00%
|
50.00%
|
| Primer 559 | Brachyspira | GCTGAAATGTCAAGCGGTGA | TCCCAATCTTCTGTGCCGT | 59.13 | 58.93 | 284 | 58 |
100.00%
|
50.00%
|
| Primer 560 | Brachyspira | AGCTGAAATGTCAAGCGGTG | TCCCAATCTTCTGTGCCGT | 59.12 | 58.93 | 285 | 58 |
100.00%
|
50.00%
|