| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 611 | Brachyspira | TGTGGCTCATGACTGCGA | TCCTACAGCTGACATACCGC | 58.94 | 59.26 | 169 | 53 |
100.00%
|
50.00%
|
| Primer 612 | Brachyspira | GGGAAATGATGAACTTGGCGA | ACGGCAGAAGCATTAGCCA | 58.91 | 59.70 | 276 | 53 |
100.00%
|
75.00%
|
| Primer 613 | Brachyspira | GGGAAATGATGAACTTGGCGA | ACGGCAGAAGCATTAGCCAT | 58.91 | 60.11 | 276 | 53 |
100.00%
|
75.00%
|
| Primer 614 | Brachyspira | TGCTTGGAGAGTTTAGTGCG | ACTCTTTGTGCCTCTCCGC | 58.20 | 60.00 | 169 | 53 |
100.00%
|
75.00%
|
| Primer 615 | Brachyspira | TTGCTTGGAGAGTTTAGTGCG | ACTCTTTGTGCCTCTCCGC | 58.86 | 60.00 | 170 | 53 |
100.00%
|
75.00%
|
| Primer 616 | Brachyspira | CCGCAGCAGGGAATAGTACA | TGCCACTCTTTGCTGCTGA | 59.54 | 59.85 | 207 | 53 |
100.00%
|
50.00%
|
| Primer 617 | Brachyspira | ACCGCAGCAGGGAATAGTAC | TGCCACTCTTTGCTGCTGA | 59.54 | 59.85 | 208 | 53 |
100.00%
|
50.00%
|
| Primer 618 | Brachyspira | GCGGTTTACGTCAGGCTGA | AGAAGCAACAGGAGCTCCG | 60.37 | 59.71 | 287 | 53 |
100.00%
|
50.00%
|
| Primer 619 | Brachyspira | GCGGTTTACGTCAGGCTGA | TAGAAGCAACAGGAGCTCCG | 60.37 | 59.47 | 288 | 53 |
100.00%
|
50.00%
|
| Primer 620 | Brachyspira | GGGGAGATGCAAGTGCTGA | TTGCTGCAGGAGAAGGCTC | 59.70 | 60.00 | 189 | 53 |
100.00%
|
50.00%
|