| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 621 | Brachyspira | GGGGAGATGCAAGTGCTGA | TCTTGCTGCAGGAGAAGGC | 59.70 | 60.00 | 191 | 53 |
100.00%
|
50.00%
|
| Primer 622 | Brachyspira | GTGCTGGTGCTATGGGGTT | GCAACAGAAACTGAAGCAGCA | 60.00 | 59.93 | 280 | 53 |
100.00%
|
50.00%
|
| Primer 623 | Brachyspira | GTGCTGGTGCTATGGGGTT | ACTGAAGCAGCAACACTCACT | 60.00 | 60.13 | 271 | 53 |
100.00%
|
50.00%
|
| Primer 624 | Brachyspira | GTGCTGGTGCTATGGGGTT | ACTGAAGCAGCAACACTCAC | 60.00 | 58.70 | 271 | 53 |
100.00%
|
50.00%
|
| Primer 625 | Brachyspira | GTGCTGGTGCTATGGGGTT | AGCAACAGAAACTGAAGCAGC | 60.00 | 59.66 | 281 | 53 |
100.00%
|
50.00%
|
| Primer 626 | Brachyspira | GGAAGTGCTTGCGACAGTG | CTCCCTTGTCCCCAAAACCT | 59.43 | 59.52 | 253 | 53 |
100.00%
|
50.00%
|
| Primer 627 | Brachyspira | GGAAGTGCTTGCGACAGTG | TCCCTTGTCCCCAAAACCT | 59.43 | 58.35 | 252 | 53 |
100.00%
|
50.00%
|
| Primer 628 | Brachyspira | ACCTTCTGGCTGCGGAAA | TCGTCCCAAATAGCAGCCT | 59.16 | 58.70 | 297 | 53 |
100.00%
|
50.00%
|
| Primer 629 | Brachyspira | GCTGATGACGGTACTACTGGA | AACCAACCGCTAGCTCCT | 58.98 | 58.19 | 218 | 53 |
100.00%
|
50.00%
|
| Primer 630 | Brachyspira | GTTGAAGCTTACGGCAGCAG | GCAATCTTAGCTTCTTCGCCT | 59.83 | 58.72 | 257 | 53 |
100.00%
|
50.00%
|