| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 641 | Brachyspira | TAATGCTGCTTGCGAGGGG | GCTTCCGCTTGTAGCAGGA | 60.45 | 60.08 | 220 | 53 |
100.00%
|
50.00%
|
| Primer 642 | Brachyspira | TAATGCTGCTTGCGAGGGG | TGCTTCCGCTTGTAGCAGG | 60.45 | 60.38 | 221 | 53 |
100.00%
|
50.00%
|
| Primer 643 | Brachyspira | CAGCAGGTTTTCTCACGCAA | AGAATGATGCCACCACCCC | 59.34 | 59.69 | 151 | 53 |
100.00%
|
50.00%
|
| Primer 644 | Brachyspira | AGTTGTTGGAAGGAGCGAGAT | ACAGGCGTTATAGCAGATGCA | 59.37 | 59.86 | 288 | 53 |
100.00%
|
50.00%
|
| Primer 645 | Brachyspira | TTGCAGCCTGCTTGGGTT | TGCTCTGAAGGCTTACCGG | 60.12 | 59.40 | 173 | 53 |
100.00%
|
50.00%
|
| Primer 646 | Brachyspira | ACGGCTGGTATATAGCACCAA | ACCATTGCCGTCTTCAGCA | 58.96 | 59.93 | 170 | 53 |
100.00%
|
50.00%
|
| Primer 647 | Brachyspira | ACGGCTGGTATATAGCACCAA | ATCCGTCAGCATGCACTCC | 58.96 | 60.15 | 216 | 53 |
100.00%
|
50.00%
|
| Primer 648 | Brachyspira | AGTTGATGAGCCTGATGGGG | AGCATAACACGTTTACCGCTT | 59.45 | 58.58 | 180 | 53 |
100.00%
|
50.00%
|
| Primer 649 | Brachyspira | AGCCTGATGGGGTTAGTGTT | AAGCATAACACGTTTACCGCT | 58.63 | 58.58 | 173 | 53 |
100.00%
|
50.00%
|
| Primer 650 | Brachyspira | AGCCTGATGGGGTTAGTGTT | CAAGCATAACACGTTTACCGC | 58.63 | 58.50 | 174 | 53 |
100.00%
|
50.00%
|