| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 651 | Brachyspira | TAGTTGATGAGCCTGATGGGG | AGCATAACACGTTTACCGCTT | 59.23 | 58.58 | 181 | 53 |
100.00%
|
50.00%
|
| Primer 652 | Brachyspira | GCCACAAACACAAACGGCA | TCTGCAGCAGTGAGAAGGG | 60.15 | 59.32 | 236 | 53 |
100.00%
|
50.00%
|
| Primer 653 | Brachyspira | GCCACAAACACAAACGGCA | TTCTGCAGCAGTGAGAAGGG | 60.15 | 59.96 | 237 | 53 |
100.00%
|
50.00%
|
| Primer 654 | Brachyspira | GCCACAAACACAAACGGCA | AGCAGTGAGAAGGGCTGTT | 60.15 | 58.85 | 231 | 53 |
100.00%
|
50.00%
|
| Primer 655 | Brachyspira | AGCTTGGGGACTTGGTTGG | TCCGTGTCCCCATTCCAA | 59.85 | 58.09 | 184 | 53 |
100.00%
|
75.00%
|
| Primer 656 | Brachyspira | AGCTTGGGGACTTGGTTGG | TTCCGTGTCCCCATTCCAA | 59.85 | 58.84 | 185 | 53 |
100.00%
|
75.00%
|
| Primer 657 | Brachyspira | TGCGAGATAGCAAGGGCA | TCGCGTACATTGTGGTCGG | 58.69 | 60.44 | 161 | 53 |
100.00%
|
100.00%
|
| Primer 658 | Brachyspira | AGGCTGCTGATGAGGCTCA | TCAAAGCCCTCATCATAGCCT | 60.99 | 58.87 | 232 | 53 |
100.00%
|
50.00%
|
| Primer 659 | Brachyspira | GGCTGAAGTTGCGGAGGTT | GCCCTTCTCTGTCTAGTAGCC | 60.60 | 59.32 | 264 | 53 |
100.00%
|
50.00%
|
| Primer 660 | Brachyspira | GGCTGAAGTTGCGGAGGTT | TCAACAGCCCTTCTCTGTCT | 60.60 | 58.28 | 270 | 53 |
100.00%
|
50.00%
|