| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 701 | Brachyspira | TGCAGGCGAAGGTGCTAA | GCCAAGAAACAGCACCGT | 59.25 | 58.57 | 173 | 50 |
100.00%
|
50.00%
|
| Primer 702 | Brachyspira | TGCAGGCGAAGGTGCTAA | TGCCAAGAAACAGCACCGT | 59.25 | 60.45 | 174 | 50 |
100.00%
|
50.00%
|
| Primer 703 | Brachyspira | TGAAGCTTACGGCAGCAGT | AGCTTCTTCGCCTCTATGTCT | 59.63 | 58.62 | 247 | 50 |
100.00%
|
50.00%
|
| Primer 704 | Brachyspira | GAGCTGAAATGTCAAGCGGTG | TCCCAATCTTCTGTGCCGT | 60.14 | 58.93 | 286 | 50 |
100.00%
|
50.00%
|
| Primer 705 | Brachyspira | TGGAGCTGAAATGTCAAGCG | TCCCAATCTTCTGTGCCGT | 58.84 | 58.93 | 288 | 50 |
100.00%
|
50.00%
|
| Primer 706 | Brachyspira | GAGCTGAAATGTCAAGCGGT | TCCCAATCTTCTGTGCCGT | 58.84 | 58.93 | 286 | 50 |
100.00%
|
50.00%
|
| Primer 707 | Brachyspira | GGCGATTCTCAAGCTAGGGC | GCCCTCTCCAAATCCACCA | 60.88 | 59.31 | 287 | 50 |
100.00%
|
50.00%
|
| Primer 708 | Brachyspira | TACCAGGTCCTTCTGCTCCA | ATCCACGCTTCTGCCCAT | 59.88 | 59.00 | 266 | 50 |
100.00%
|
50.00%
|
| Primer 709 | Brachyspira | GGTCCTGCACGTGCTGTAT | AGCCCTCCCATAAATCTGGTC | 60.08 | 59.22 | 180 | 50 |
100.00%
|
100.00%
|
| Primer 710 | Brachyspira | GGTCCTGCACGTGCTGTAT | AGCCCTCCCATAAATCTGGT | 60.08 | 58.09 | 180 | 50 |
100.00%
|
100.00%
|