| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 721 | Brachyspira | TGGTTCGGAATAGGGGAGGT | ACCCAGCCTACACCAGCAA | 59.96 | 61.15 | 278 | 49 |
100.00%
|
50.00%
|
| Primer 722 | Brachyspira | TGGTTCGGAATAGGGGAGGT | AACCCAGCCTACACCAGCA | 59.96 | 61.15 | 279 | 49 |
100.00%
|
50.00%
|
| Primer 723 | Brachyspira | TGCCAGATGCGGTTATGCT | TAACGCCTCTTGCCTCTGC | 59.78 | 60.08 | 233 | 49 |
100.00%
|
75.00%
|
| Primer 724 | Brachyspira | AGCGGATCAAAAGCGGATTT | ACGAATGCCATTAATGCCAGA | 58.54 | 58.35 | 200 | 49 |
100.00%
|
50.00%
|
| Primer 725 | Brachyspira | TAGCGGATCAAAAGCGGATTT | ACGAATGCCATTAATGCCAGA | 58.36 | 58.35 | 201 | 49 |
100.00%
|
50.00%
|
| Primer 726 | Brachyspira | GGTATAGGTCGCACTCTTGC | CGCTGACCATGTACCGGAA | 58.15 | 59.78 | 236 | 49 |
100.00%
|
50.00%
|
| Primer 727 | Brachyspira | TGGGGAGATGCAAGTGCTG | TTGCTGCAGGAGAAGGCT | 60.00 | 58.84 | 190 | 49 |
100.00%
|
50.00%
|
| Primer 728 | Brachyspira | GGGGAGATGCAAGTGCTGA | CTTGCTGCAGGAGAAGGCT | 59.70 | 60.00 | 190 | 49 |
100.00%
|
50.00%
|
| Primer 729 | Brachyspira | TTGGAAGGGCTTGCTGCT | AGCACCGCATACAGCACAT | 59.48 | 60.08 | 294 | 49 |
100.00%
|
75.00%
|
| Primer 730 | Brachyspira | GCAATGGTTCGTCCTAGGGG | TCGCCTCCGGAAGTGAGTA | 60.46 | 60.00 | 287 | 48 |
100.00%
|
75.00%
|