| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 731 | Brachyspira | TGTCCGCAAAGAGAAGGGG | ACCTGTAGCTTCACGGCT | 59.63 | 58.21 | 240 | 48 |
100.00%
|
50.00%
|
| Primer 732 | Brachyspira | CCATTGCCTACAAGCATACGT | ACATCAACCCCAGCTGGAA | 58.71 | 58.84 | 173 | 48 |
100.00%
|
50.00%
|
| Primer 733 | Brachyspira | CCATTGCCTACAAGCATACGT | AACATCAACCCCAGCTGGA | 58.71 | 58.84 | 174 | 48 |
100.00%
|
50.00%
|
| Primer 734 | Brachyspira | GGTCCTGCACGTGCTGTAT | CTGCCCTCCCATAAATCTGGT | 60.08 | 59.50 | 181 | 48 |
100.00%
|
100.00%
|
| Primer 735 | Brachyspira | GGTCCTGCACGTGCTGTAT | TGCCCTCCCATAAATCTGGT | 60.08 | 58.39 | 180 | 48 |
100.00%
|
100.00%
|
| Primer 736 | Brachyspira | GGTCCTGCACGTGCTGTAT | TCTGCCCTCCCATAAATCTGG | 60.08 | 59.22 | 182 | 48 |
100.00%
|
100.00%
|
| Primer 737 | Brachyspira | GGTCCTGCACGTGCTGTAT | GCCCTCCCATAAATCTGGTCT | 60.08 | 59.22 | 179 | 48 |
100.00%
|
100.00%
|
| Primer 738 | Brachyspira | TGACGGCTCTGTGGGTGAT | ACTCCGCATGCCATTCTCT | 60.91 | 59.09 | 183 | 48 |
100.00%
|
75.00%
|
| Primer 739 | Brachyspira | ATGACGGCTCTGTGGGTGA | ACTCCGCATGCCATTCTCT | 60.91 | 59.09 | 184 | 48 |
100.00%
|
75.00%
|
| Primer 740 | Brachyspira | TGATGACGGCTCTGTGGGT | ACTCCGCATGCCATTCTCT | 60.91 | 59.09 | 186 | 48 |
100.00%
|
50.00%
|