| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 861 | Brachyspira | GCTTGGAGAGTTTAGTGCGG | CGCCAGAAAGCTCCCCTAT | 58.92 | 59.17 | 152 | 26 |
100.00%
|
75.00%
|
| Primer 862 | Brachyspira | AAACGGAGAGGAAGGCGT | GCACCTGCTAGAAGGGCTTT | 58.53 | 60.32 | 192 | 26 |
100.00%
|
50.00%
|
| Primer 863 | Brachyspira | TGCCAGATGGAAGCGTATCA | CCTTCAAAAGCAGGTCCGC | 59.17 | 59.42 | 298 | 25 |
100.00%
|
50.00%
|
| Primer 864 | Brachyspira | TGGACCAAGAAACTGGCGA | TGAGCTGTATCCCTTGCCG | 59.17 | 59.48 | 280 | 24 |
100.00%
|
50.00%
|
| Primer 865 | Brachyspira | TGGACCAAGAAACTGGCGA | GAGCTGTATCCCTTGCCGT | 59.17 | 59.48 | 279 | 24 |
100.00%
|
50.00%
|
| Primer 866 | Brachyspira | TGTAGTTGTTGGAAGGAGCGA | ACAGGCGTTATAGCAGATGCA | 59.31 | 59.86 | 291 | 24 |
100.00%
|
50.00%
|
| Primer 867 | Brachyspira | ACCCCTGTTGTTGTACAGCT | TGCTGCTTCCATCTGTCCT | 59.16 | 58.62 | 177 | 22 |
100.00%
|
50.00%
|
| Primer 868 | Brachyspira | ACAGGGGTACTTGAAAGAGCT | TGCTTCGCCTTCGCCATAA | 58.66 | 60.08 | 270 | 21 |
100.00%
|
50.00%
|
| Primer 869 | Brachyspira | GGTCCTGCACGTGCTGTATT | TGCCCTCCCATAAATCTGGTC | 60.67 | 59.51 | 180 | 19 |
100.00%
|
100.00%
|
| Primer 870 | Brachyspira | ACGACAGTGCTTGGAACGA | AGGAGCAAACTCTTTCGCCA | 59.56 | 59.89 | 218 | 17 |
100.00%
|
75.00%
|