| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1951 | Brevibacillus | GGGTTTTGCGCTTGCCTT | TTGCAATTGCCGTAGCGC | 59.58 | 59.82 | 152 | 65 |
100.00%
|
50.00%
|
| Primer 1952 | Brevibacillus | TTTGGCAGTCGTCCGCAA | TGCAGCTCGCGAAGTCTT | 60.20 | 59.66 | 174 | 65 |
100.00%
|
50.00%
|
| Primer 1953 | Brevibacillus | TTTTGGCAGTCGTCCGCA | TGCAGCTCGCGAAGTCTT | 60.20 | 59.66 | 175 | 65 |
100.00%
|
50.00%
|
| Primer 1954 | Brevibacillus | AGGGCAGCTTCGTTTCCA | TGGCTTTCGCTGCTGACA | 59.16 | 59.89 | 240 | 65 |
100.00%
|
50.00%
|
| Primer 1955 | Brevibacillus | ACGACCCGATTCTTGCCA | AGCCTTTGCGACCTGCTT | 58.93 | 59.89 | 297 | 65 |
100.00%
|
50.00%
|
| Primer 1956 | Brevibacillus | TGGCACAGGGATTACACGC | AACGCATCCAACGCCTGT | 60.38 | 60.28 | 202 | 65 |
100.00%
|
50.00%
|
| Primer 1957 | Brevibacillus | TTTTCGTTGCGCTTGGGC | TGTCCAGCCAGCTGCATAC | 59.97 | 60.08 | 230 | 65 |
100.00%
|
50.00%
|
| Primer 1958 | Brevibacillus | TGCATGTTCCTCGCGAGT | TCACGGTGCCACTTGCTT | 59.34 | 59.81 | 165 | 65 |
100.00%
|
50.00%
|
| Primer 1959 | Brevibacillus | CGGCTGGAAAACCTGGGAT | TCACGGTGCCACTTGCTT | 60.00 | 59.81 | 198 | 65 |
100.00%
|
50.00%
|
| Primer 1960 | Brevibacillus | AATTCGCTTGAGCACGCC | CACGCAATCGAGGGACCTT | 59.13 | 60.08 | 205 | 65 |
100.00%
|
50.00%
|