| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1961 | Brevibacillus | TTGGCTTCCACACGGCTT | TGCTCGAGCCATGGACAA | 59.81 | 58.93 | 183 | 65 |
100.00%
|
50.00%
|
| Primer 1962 | Brevibacillus | ACCCGAAAATGGCTCGCT | TGACCTGTGCGTTCTGCTT | 59.65 | 59.85 | 196 | 65 |
100.00%
|
50.00%
|
| Primer 1963 | Brevibacillus | GAAAATCGCTTCCCGTCGC | TTTGCTCCTGGCTGCATGA | 59.94 | 59.92 | 234 | 65 |
100.00%
|
50.00%
|
| Primer 1964 | Brevibacillus | GAAAATCGCTTCCCGTCGC | CCTGGCTGCATGACATCCA | 59.94 | 60.08 | 228 | 65 |
100.00%
|
50.00%
|
| Primer 1965 | Brevibacillus | ACCGCAAACACACAGCCA | TTCGCAAACCGCATGCAG | 60.44 | 59.75 | 273 | 65 |
100.00%
|
50.00%
|
| Primer 1966 | Brevibacillus | ACCGCAAACACACAGCCA | CTTCGCAAACCGCATGCA | 60.44 | 59.75 | 274 | 65 |
100.00%
|
50.00%
|
| Primer 1967 | Brevibacillus | CGGACTGACGCTCGTTCTA | TGATCAGATTGCCGTCGGG | 59.21 | 59.86 | 191 | 65 |
100.00%
|
50.00%
|
| Primer 1968 | Brevibacillus | TGTGCCAAAGGTTGCCCA | TGTCCACGAATCGCAGCA | 60.04 | 59.66 | 195 | 65 |
100.00%
|
50.00%
|
| Primer 1969 | Brevibacillus | ATCGTCAATGCCAACCGC | TGGCTGTACCGCTGACAA | 58.81 | 58.86 | 174 | 65 |
100.00%
|
50.00%
|
| Primer 1970 | Brevibacillus | CAACAGCTCATGCGTGCTT | AAGGTTTCGTGCAGCGGT | 59.42 | 60.20 | 239 | 65 |
100.00%
|
50.00%
|