| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 4161 | Brevibacillus | TTGGTCTGGCTGCATTGGG | ACTGCGATGATCGGCACA | 60.61 | 59.42 | 154 | 47 |
100.00%
|
50.00%
|
| Primer 4162 | Brevibacillus | TCGCTTGTTTGCATCGCG | AGTCCTGCAGCAGTAGGGA | 59.83 | 59.92 | 181 | 47 |
100.00%
|
50.00%
|
| Primer 4163 | Brevibacillus | TCATGCAGCAGCTCAGCA | AGAAACGTGCCGAGCAGT | 59.65 | 59.58 | 163 | 47 |
100.00%
|
50.00%
|
| Primer 4164 | Brevibacillus | AACGGGTGGATTTGCGCA | ATGCGGTTTGTTGTGGGC | 60.60 | 59.27 | 206 | 47 |
100.00%
|
50.00%
|
| Primer 4165 | Brevibacillus | AAACGTCGGCTTCGGCA | TTCCGTCGTTGTCGTGCA | 59.93 | 59.90 | 188 | 47 |
100.00%
|
50.00%
|
| Primer 4166 | Brevibacillus | TCGAAGCGCTGAACAGAGG | TGATCGCTTTCGCCAGCA | 60.08 | 60.05 | 285 | 47 |
100.00%
|
50.00%
|
| Primer 4167 | Brevibacillus | TGCTGGGTGTGGCCTTTT | TGCGAACCATCAGGCAGT | 59.72 | 59.25 | 213 | 47 |
100.00%
|
50.00%
|
| Primer 4168 | Brevibacillus | AGCGCTGGTGGAAATTGC | AAAAGGCCACACCCAGCA | 59.04 | 59.72 | 215 | 47 |
100.00%
|
50.00%
|
| Primer 4169 | Brevibacillus | GCTTTGCGGTTTGACGAGG | AAAAGGCCACACCCAGCA | 60.08 | 59.72 | 271 | 47 |
100.00%
|
50.00%
|
| Primer 4170 | Brevibacillus | AACGGTTGAGCAGGGCTA | GCGAGCGTGTTTGCCTTT | 58.52 | 59.67 | 217 | 47 |
100.00%
|
50.00%
|