| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 4181 | Brevibacillus | TGAGCAATCGTCCGCACT | TGTGGTGGGTGTTATGGCC | 59.34 | 59.92 | 159 | 47 |
100.00%
|
50.00%
|
| Primer 4182 | Brevibacillus | AACACCGTCCTTGCCGAT | TTGACCAGCACTTCGGCA | 59.25 | 59.49 | 185 | 47 |
100.00%
|
50.00%
|
| Primer 4183 | Brevibacillus | TTTGACGGCAAGCTGGCA | TGTCTTCCCGATACGTGCG | 60.52 | 59.86 | 160 | 47 |
100.00%
|
50.00%
|
| Primer 4184 | Brevibacillus | TTTTGACGGCAAGCTGGC | TGTCTTCCCGATACGTGCG | 59.27 | 59.86 | 161 | 47 |
100.00%
|
50.00%
|
| Primer 4185 | Brevibacillus | TGCCAAAACCGCTGTGGA | TGGCGTGATCGTAGTGCA | 60.12 | 59.03 | 264 | 47 |
100.00%
|
50.00%
|
| Primer 4186 | Brevibacillus | AGCTTTTCGGCTTGTGCG | AACCTGTCGGCAGCGAAA | 59.36 | 59.89 | 172 | 47 |
100.00%
|
50.00%
|
| Primer 4187 | Brevibacillus | ACTATGCAGGGTCGTGTCG | CCTTGGAAAGCGGACGAGT | 59.49 | 60.00 | 190 | 47 |
100.00%
|
50.00%
|
| Primer 4188 | Brevibacillus | ACGCAACAAGGACGGCAT | AACAGCTGCGCTTCTGCA | 60.28 | 60.59 | 291 | 47 |
100.00%
|
50.00%
|
| Primer 4189 | Brevibacillus | ACTGCCGTCAACCAGCTAC | TGACCAGGGAACAAGCTCG | 60.00 | 59.63 | 170 | 47 |
100.00%
|
50.00%
|
| Primer 4190 | Brevibacillus | AACATCGACCACCTGAGCG | TCCATCCTCCTCCGCTTCT | 60.08 | 59.69 | 215 | 47 |
100.00%
|
50.00%
|