| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 121 | Clostridioides | TCCAGGAGATAAAGGCGATGT | CTGGACAATGTGCATCTGAGC | 58.60 | 59.60 | 170 | 76 |
100.00%
|
69.23%
|
| Primer 122 | Clostridioides | TCCAGGAGATAAAGGCGATGT | TGGACAATGTGCATCTGAGC | 58.60 | 58.54 | 169 | 76 |
100.00%
|
69.23%
|
| Primer 123 | Clostridioides | ACTGTTGAGGCTCATTGGC | TTTCCGCTTCCACATGGGC | 58.05 | 60.98 | 158 | 76 |
100.00%
|
61.54%
|
| Primer 124 | Clostridioides | GACTCTACGGCACATGCAGA | TGATCGAGCATTCTTCTTGCA | 59.83 | 58.02 | 274 | 75 |
100.00%
|
61.54%
|
| Primer 125 | Clostridioides | ACTCTACGGCACATGCAGA | TGATCGAGCATTCTTCTTGCA | 58.72 | 58.02 | 273 | 75 |
100.00%
|
61.54%
|
| Primer 126 | Clostridioides | TGCCCATACTGATTTGAGGGA | ACCTGTTCCTGGAAACACAGA | 58.80 | 59.16 | 283 | 75 |
100.00%
|
61.54%
|
| Primer 127 | Clostridioides | AGCGCAAGGAAGCAGAAGA | TGCCTCATCAAACTCCTCAAC | 59.63 | 58.22 | 164 | 75 |
100.00%
|
61.54%
|
| Primer 128 | Clostridioides | GCGCAAGGAAGCAGAAGA | TGCCTCATCAAACTCCTCAAC | 58.03 | 58.22 | 163 | 75 |
100.00%
|
61.54%
|
| Primer 129 | Clostridioides | AAGCGCAAGGAAGCAGAAGA | TGCCTCATCAAACTCCTCAAC | 60.25 | 58.22 | 165 | 75 |
100.00%
|
61.54%
|
| Primer 130 | Clostridioides | TTAGGGCTCGCAACTCGTG | TCCTACAACTGCTCTTGGAGA | 60.08 | 58.11 | 228 | 75 |
100.00%
|
69.23%
|