| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 141 | Clostridioides | TAGGTGCTGGGCTTGTTGG | GTAGAAGCTGAGCCACCCA | 59.93 | 59.02 | 265 | 74 |
100.00%
|
61.54%
|
| Primer 142 | Clostridioides | TCTTAGGGCTCGCAACTCG | TCCTACAACTGCTCTTGGAGA | 59.49 | 58.11 | 230 | 74 |
100.00%
|
69.23%
|
| Primer 143 | Clostridioides | TTCTTAGGGCTCGCAACTCG | TCCTACAACTGCTCTTGGAGA | 60.11 | 58.11 | 231 | 74 |
100.00%
|
69.23%
|
| Primer 144 | Clostridioides | TTGACTGATGCAGGTTCACC | CCTACCCAACTGTATGCCCA | 58.10 | 59.08 | 188 | 74 |
100.00%
|
61.54%
|
| Primer 145 | Clostridioides | TTTGGCAGTTGAAGTGTGGAT | TGCTTCGCGAATTGCTCC | 58.33 | 58.82 | 154 | 74 |
100.00%
|
61.54%
|
| Primer 146 | Clostridioides | ACCTAGAAGTGGGCTTGCC | TCCAGTTGAGCCAAACCCA | 59.31 | 59.07 | 244 | 74 |
100.00%
|
61.54%
|
| Primer 147 | Clostridioides | GCAGATGGCGTTGTAACTCC | TGTCCCCATCACTGAACCA | 59.27 | 58.14 | 225 | 74 |
100.00%
|
61.54%
|
| Primer 148 | Clostridioides | GTTGCTGTTGATGCCGACC | CCACCAGAATCTACGGTTCCC | 59.79 | 60.13 | 245 | 73 |
100.00%
|
61.54%
|
| Primer 149 | Clostridioides | TTGACTGATGCAGGTTCACC | ACCTACCCAACTGTATGCCC | 58.10 | 59.08 | 189 | 73 |
100.00%
|
61.54%
|
| Primer 150 | Clostridioides | ATTTGGCAGTTGAAGTGTGGA | TGCTTCGCGAATTGCTCC | 58.33 | 58.82 | 155 | 73 |
100.00%
|
61.54%
|